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Pathogens

Title:		Biological Control of Tomato Gray Mold Caused by Botrytis Cinerea with the Entomopathogenic Fungus Metarhizium Anisopliae
Author(s):		Sarven MS; Hao Q; Deng J; Yang F; Wang G; Xiao Y; Xiao X;
Address:		"Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Jingmen (China Valley) Academy of Agricultural Science, Jingmen 448000, Hubei, China"
Journal Title:		Pathogens
Year:		2020
Volume:		20200313
Issue:		3
Page Number:		-
DOI:		10.3390/pathogens9030213
ISSN/ISBN:		2076-0817 (Print) 2076-0817 (Electronic) 2076-0817 (Linking)
Abstract:		"Gray mold disease caused by Botrytis cinerea is a devastating disease that leads to serious financial loss. In this study, the entomopathogenic fungus Metarhizium anisopliae that acts against the gray mold pathogen B. cinerea was evaluated. M. anisopliae produced a significant inhibition zone in front of the B. cinerea colony in the dual culture test. In addition, volatile organic compounds generated by M. anisopliae were shown to have an inhibitory effect on B. cinerea mycelia growth and reduced 41% of gray mold severity of postharvest tomatoes. The 10% concentration of the culture filtrate of M. anisopliae inhibited 88.62% of colony radial growth as well as 63.85% of sclerotia germination and all conidia germination of B. cinerea. Furthermore, the culture filtrate of M. anisopliae retained its inhibitory effect against the radial growth of B. cinerea even after heating for 15 min at 100 degrees C. Feasible mechanisms of M. anisopliae involved in the control of B. cinerea were explored, and it was demonstrated that the plasma membrane of B. cinerea conidia was damaged by the product of metabolism of M. anisopliae. In addition, after treating with culture filtrate of M. anisopliae, the B. cinerea phenotype was shown to be abnormal, and cell organelles of B. cinerea mycelia were damaged significantly. A significant control efficacy of M. anisopliae against tomato gray mold was detected on both the detached leaf assay (84.24%) as well as the whole plant (72.38%). In addition, a 78% reduction in tomato fruit mold was detected at a 10% treated concentration of M. anisopliae. These findings suggest that M. anisopliae possesses potential as a biocontrol agent against tomato gray mold in the greenhouse and during the postharvest stage"
Keywords:		Botrytis cinerea Metarhizium anisopliae biocontrol conidia gray mold postharvest;
Notes:		"PubMed-not-MEDLINESarven, Most Sinthia Hao, Qiuyan Deng, Junbo Yang, Fang Wang, Gaofeng Xiao, Yannong Xiao, Xueqiong eng 31501595/National Natural Science Foundation of China/ 2018YFD0201006/National Key R&D Program of China/ Switzerland 2020/03/19 Pathogens. 2020 Mar 13; 9(3):213. doi: 10.3390/pathogens9030213"