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Methods Mol Biol

Title:		Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry
Author(s):		Neag EJ; Collao V; Bhattacharya SK;
Address:		"Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA. Miami Integrative Metabolomics Research Center, Miami, FL, USA. Michigan State University College of Osteopathic Medicine, East Lansing, MI, USA. Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA. neagemil@msu.edu. Miami Integrative Metabolomics Research Center, Miami, FL, USA. neagemil@msu.edu"
Journal Title:		Methods Mol Biol
Year:		2023
Volume:		2625
Issue:
Page Number:		141 - 148
DOI:		10.1007/978-1-0716-2966-6_13
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"An optimized Bligh and Dyer protocol and subsequent derivatization is described in this chapter for the extraction of free cholesterol and cholesterol esters from tissue samples. Quantification analysis of lipid species is then described utilizing gas chromatography-mass spectrometry, the ideal method for analysis of volatile organic compounds and extraction of sterols"
Keywords:		Gas Chromatography-Mass Spectrometry/methods *Cholesterol Sterols/analysis Cholesterol Esters *Phytosterols Bligh and Dyer extraction Cholesterol Derivatization Gas chromatography-mass spectrometry Lipidomic Optic tissue;
Notes:		"MedlineNeag, Emily J Collao, Vanessa Bhattacharya, Sanjoy K eng 2023/01/19 Methods Mol Biol. 2023; 2625:141-148. doi: 10.1007/978-1-0716-2966-6_13"