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Proc Natl Acad Sci U S A

Title:		Active conformation of an insect neuropeptide family
Author(s):		Nachman RJ; Roberts VA; Dyson HJ; Holman GM; Tainer JA;
Address:		"Veterinary Toxicology and Entomology Research Laboratory, U.S. Department of Agriculture, College Station, TX 77840"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1991
Volume:		88
Issue:		10
Page Number:		4518 - 4522
DOI:		10.1073/pnas.88.10.4518
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"To understand the structural and chemical basis for insect neuropeptide activity, we have designed, synthesized, and determined the conformation of a biologically active cyclic analog of the pyrokinins, an insect neuropeptide family that mediates myotropic (visceral muscle contractile) activity. Members of this insect neuropeptide family share the common C-terminal pentapeptide sequence Phe-Xaa-Pro-Arg-Leu-NH2 (Xaa = Ser, Thr, or Val). Circular dichroic, nuclear magnetic resonance, and molecular dynamics analyses of the conformationally restricted cyclic pyrokinin analog cyclo(-Asn-Thr-Ser-Phe-Thr-Pro-Arg-Leu-) indicated the presence of a beta-turn in the active core region encompassing residues Thr-Pro-Arg-Leu. The rigid cyclic analog retains biological activity, suggesting that its C-terminal beta-turn is the active pyrokinin conformation recognized by the myotropic receptor. As individual pyrokinins and pyrokinin-like neuropeptides demonstrate both oviduct-contractile and pheromone-biosynthesis activities in various insects, the biologically active beta-turn structure reported here holds broad significance for many biological processes"
Keywords:		"Amino Acid Sequence Animals Circular Dichroism Cockroaches/*analysis Magnetic Resonance Spectroscopy Molecular Sequence Data Molecular Structure Neuropeptides/chemical synthesis/*chemistry Peptides, Cyclic/chemical synthesis/*chemistry Protein Conformatio;"
Notes:		"MedlineNachman, R J Roberts, V A Dyson, H J Holman, G M Tainer, J A eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 1991/05/15 Proc Natl Acad Sci U S A. 1991 May 15; 88(10):4518-22. doi: 10.1073/pnas.88.10.4518"