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Talanta

Title:		Assessment of gas chromatography time-of-flight accurate mass spectrometry for identification of volatile and semi-volatile compounds in honey
Author(s):		Moniruzzaman M; Rodriguez I; Ramil M; Cela R; Sulaiman SA; Gan SH;
Address:		"Departamento de Quimica Analitica, Nutricion y Bromatologia, Instituto de Investigacion y Analisis Alimentario (IIAA), Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain; Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Departamento de Quimica Analitica, Nutricion y Bromatologia, Instituto de Investigacion y Analisis Alimentario (IIAA), Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain. Electronic address: isaac.rodriguez@usc.es. Departamento de Quimica Analitica, Nutricion y Bromatologia, Instituto de Investigacion y Analisis Alimentario (IIAA), Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain. Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia"
Journal Title:		Talanta
Year:		2014
Volume:		20140618
Issue:
Page Number:		505 - 515
DOI:		10.1016/j.talanta.2014.06.019
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"The performance of gas chromatography (GC) combined with a hybrid quadrupole time-of-flight (QTOF) mass spectrometry (MS) system for the determination of volatile and semi-volatile compounds in honey samples is evaluated. After headspace (HS) solid-phase microextraction (SPME) of samples, the accurate mass capabilities of the above system were evaluated for compounds identification. Accurate scan electron impact (EI) MS spectra allowed discriminating compounds displaying the same nominal masses, but having different empirical formulae. Moreover, the use of a mass window with a width of 0.005 Da provided highly specific chromatograms for selected ions, avoiding the contribution of interferences to their peak areas. Additional information derived from positive chemical ionization (PCI) MS spectra and ion product scan MS/MS spectra permitted confirming the identity of novel compounds. The above possibilities are illustrated with examples of honey aroma compounds, belonging to different chemical classes and containing different elements in their molecules. Examples of compounds whose structures could not be described are also provided. Overall, 84 compounds, from a total of 89 species, could be identified in 19 honey samples from 3 different geographic areas in the world. The suitability of responses measured for selected ions, corresponding to above species, for authentication purposes is assessed through principal components analysis"
Keywords:		Alkaloids/chemistry Benzene/chemistry Carboxylic Acids/chemistry Esters/chemistry Food Analysis/*methods Gas Chromatography-Mass Spectrometry/*methods Honey/*analysis Ions Molecular Weight Principal Component Analysis Quinolines/chemistry Reproducibility;
Notes:		"MedlineMoniruzzaman, M Rodriguez, I Ramil, M Cela, R Sulaiman, S A Gan, S H eng Research Support, Non-U.S. Gov't Netherlands 2014/08/17 Talanta. 2014 Nov; 129:505-15. doi: 10.1016/j.talanta.2014.06.019. Epub 2014 Jun 18"