| Title: | Odorant binding characteristics of three recombinant odorant binding proteins in Microplitis mediator (Hymenoptera: Braconidae) |
| Author(s): | Li K; Wang S; Zhang K; Ren L; Ali A; Zhang Y; Zhou J; Guo Y; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China" |
| DOI: | 10.1007/s10886-014-0458-5 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. In this study, three new putative OBP genes, MmedOBP8-10, were identified from a Microplitis mediator (Hymenoptera: Braconidae) antennal cDNA library. Quantitative real-time PCR (qRT-PCR) analysis revealed that all three of the OBP genes were expressed mainly in the antennae of adult wasps. The three OBPs were recombinantly expressed in Escherichia coli and purified by Ni ion affinity chromatography. Fluorescence competitive binding assays were performed using N-phenyl-naphthylamine as a fluorescent probe and 45 small organic compounds as competitors. These assays demonstrated that the three M. mediator OBPs can bind a broad range of odorant molecules with different binding affinities. They can bind the following ligands: nonane, farnesol, nerolidol, nonanal, beta-ionone, acetic ether, and farnesene. In a Y-tube assay with these ligands as odor stimuli and paraffin oil as a control, all ligands, except nerolidol and acetic ether, were able to elicit behavioral responses in adult M. mediator. The wasps were significantly attracted to beta-ionone, nonanal, and farnesene and repelled by nonane and farnesol. The results of this work provide insight into the chemosensory functions of the OBPs in M. mediator" |
| Keywords: | "1-Naphthylamine/analogs & derivatives/metabolism Aldehydes Animals Arthropod Antennae Behavior, Animal Female Fluorescent Dyes/metabolism Gene Expression Regulation *Hymenoptera/genetics Insect Proteins/genetics/*metabolism Male Molecular Sequence Data No;" |
| Notes: | "MedlineLi, Keming Wang, Shanning Zhang, Kang Ren, Liyan Ali, Abid Zhang, Yongjun Zhou, Jingjiang Guo, Yuyuan eng Research Support, Non-U.S. Gov't 2014/06/15 J Chem Ecol. 2014 Jun; 40(6):541-8. doi: 10.1007/s10886-014-0458-5. Epub 2014 Jun 14" |
