« Previous Abstract"Identification of volatile and nonvolatile compounds in Citri Reticulatae Pericarpium Viride using GC-MS, UPLC-Q-Exactive Orbitrap-MS, and HPLC-PDA"    Next AbstractIntegration of Volatilomics and Metabolomics Unveils Key Flavor-Related Biological Pathways in Different Carambola Cultivars »

J Agric Food Chem

Title:		Inducible Gut-Specific Carboxylesterase SlCOE030 in Polyphagous Pests of Spodoptera litura Conferring Tolerance between Nicotine and Cyantraniliprole
Author(s):		Li J; Lv Y; Liu Y; Bi R; Pan Y; Shang Q;
Address:		"College of Plant Science, Jilin University, Changchun 130062, P. R. China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, P. R. China"
Journal Title:		J Agric Food Chem
Year:		2023
Volume:		20230306
Issue:		10
Page Number:		4281 - 4291
DOI:		10.1021/acs.jafc.3c00524
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Insecticides tolerance in herbivorous arthropods is associated with preadaptation to host plant allelochemicals. However, how plant secondary metabolites activate detoxifying metabolic genes to develop tolerance remains unclear. Herein, the tolerance of Spodoptera litura larvae to cyantraniliprole was increased after nicotine exposure. An S. litura alpha esterase, SlCOE030, was predominantly expressed in the midgut and induced after exposure to cyantraniliprole, nicotine, and cyantraniliprole plus nicotine. Drosophila melanogaster with ectopically overexpressed SlCOE030 enhanced cyantraniliprole and nicotine tolerance by 4.91- and 2.12-fold, respectively. Compared to UAS-SlCOE030 and Esg-GAL4 lines, the Esg > SlCOE030 line laid more eggs after nicotine exposure. SlCOE030 knockdown decreased the sensitivity of nicotine-treated S. litura larvae to cyantraniliprole. Metabolism assays indicated that recombinant SlCOE030 protein metabolizes cyantraniliprole. Homology modeling and molecular docking analysis demonstrated that SlCOE030 exhibits effective affinities for cyantraniliprole and nicotine. Thus, insect CarEs may result in the development of cross-tolerance between synthetic insecticides and plant secondary metabolites"
Keywords:		Animals *Insecticides/pharmacology Nicotine/pharmacology Spodoptera Carboxylesterase/genetics Drosophila melanogaster Molecular Docking Simulation Larva/genetics Sicoe030 Spodoptera litura cyantraniliprole gut-specific nicotine;
Notes:		"MedlineLi, Jianyi Lv, Yuntong Liu, Yajing Bi, Rui Pan, Yiou Shang, Qingli eng 2023/03/07 J Agric Food Chem. 2023 Mar 15; 71(10):4281-4291. doi: 10.1021/acs.jafc.3c00524. Epub 2023 Mar 6"