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J Biol Chem

Title:		"Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades"
Author(s):		Hutchison M; Berman KS; Cobb MH;
Address:		"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9041, USA"
Journal Title:		J Biol Chem
Year:		1998
Volume:		273
Issue:		44
Page Number:		28625 - 28632
DOI:		10.1074/jbc.273.44.28625
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Several components of the budding yeast pheromone-response pathway are conserved in mammalian mitogen-activated protein (MAP) kinase pathways. Thus, we used degenerate oligonucleotides derived from the sequence of the Saccharomyces cerevisiae protein kinase Ste20p to amplify related sequences from the rat. One of these sequences was used to clone a rat Ste20p homolog, which we called TAO1 for its one thousand and one amino acids. Northern analysis shows TAO1 is highly expressed in brain, as is a homolog TAO2. Recombinant TAO1 was expressed and purified from Sf9 cells. In vitro, it activated MAP/extracellular signal-regulated protein kinase (ERK) kinases (MEKs) 3, 4, and 6 of the stress-responsive MAP kinase pathways, but not MEK1 or 2 of the classical MAP kinase pathway. TAO1 activated MEK3 but not MEK4 or MEK6 in transfected cells. MEK3 coimmunoprecipitated with TAO1 when they were expressed in 293 cells. In addition, immunoreactive MEK3 endogenous to Sf9 cells copurified with TAO1 produced from a recombinant baculovirus. The activation of and binding to MEK3 by TAO1 implicates TAO1 in the regulation of the p38-containing stress-responsive MAP kinase pathway"
Keywords:		"Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases/metabolism Cell Line Cloning, Molecular DNA, Complementary Enzyme Activation Humans MAP Kinase Kinase 3 *MAP Kinase Kinase 4 MAP Kinase Kinase 6 *MAP Kinase Kinase Kina;"
Notes:		"MedlineHutchison, M Berman, K S Cobb, M H eng DK34128/DK/NIDDK NIH HHS/ GM53032/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1998/10/24 J Biol Chem. 1998 Oct 30; 273(44):28625-32. doi: 10.1074/jbc.273.44.28625"