| Title: | Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda |
| Author(s): | Hafeez M; Li X; Chen L; Ullah F; Huang J; Zhang Z; Zhang J; Siddiqui JA; Zhou SX; Ren XY; Imran M; Assiri MA; Lou Y; Lu Y; |
| Address: | "State Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. Integrated Plant Protection Center, Lishui Academy of Agricultural and Forestry Sciences, Lishui, China. Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, China. College of Agriculture, College of Tobacco Science, Guizhou University, Guiyang, China. Department of Chemistry, Faculty of Science, King Khalid University, Abha, Saudi Arabia" |
| DOI: | 10.3389/fpls.2022.1079442 |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "The fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene CYP302A1 and how S. frugieprda larvae use a detoxification mechanism to adapt host plants. Results demonstrated that CYP302A1 expression levels were much higher in midgut tissue and the older S. frugiperda larvae. Our current studies revealed the enhanced P450 activity in the midguts of S. frugiperda larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants" |
| Keywords: | CYP302a1 P450 detoxification enzyme RNAi host adaptation spodoptera frugiperda; |
| Notes: | "PubMed-not-MEDLINEHafeez, Muhammad Li, Xiaowei Chen, Limin Ullah, Farman Huang, Jun Zhang, Zhijun Zhang, Jinming Siddiqui, Junaid Ali Zhou, Shu-Xing Ren, Xiao-Yun Imran, Muhammad Assiri, Mohammed A Lou, Yonggen Lu, Yaobin eng Switzerland 2023/02/11 Front Plant Sci. 2023 Jan 25; 13:1079442. doi: 10.3389/fpls.2022.1079442. eCollection 2022" |
