| Title: | Engineering fungal de novo fatty acid synthesis for short chain fatty acid production |
| Author(s): | Gajewski J; Pavlovic R; Fischer M; Boles E; Grininger M; |
| Address: | "Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence 'Macromolecular Complexes', Goethe University Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt, Germany. Institute of Molecular Biosciences, Department of Biological Sciences, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt, Germany" |
| ISSN/ISBN: | 2041-1723 (Electronic) 2041-1723 (Linking) |
| Abstract: | "Fatty acids (FAs) are considered strategically important platform compounds that can be accessed by sustainable microbial approaches. Here we report the reprogramming of chain-length control of Saccharomyces cerevisiae fatty acid synthase (FAS). Aiming for short-chain FAs (SCFAs) producing baker's yeast, we perform a highly rational and minimally invasive protein engineering approach that leaves the molecular mechanisms of FASs unchanged. Finally, we identify five mutations that can turn baker's yeast into a SCFA producing system. Without any further pathway engineering, we achieve yields in extracellular concentrations of SCFAs, mainly hexanoic acid (C(6)-FA) and octanoic acid (C(8)-FA), of 464 mg l(-1) in total. Furthermore, we succeed in the specific production of C(6)- or C(8)-FA in extracellular concentrations of 72 and 245 mg l(-1), respectively. The presented technology is applicable far beyond baker's yeast, and can be plugged into essentially all currently available FA overproducing microorganisms" |
| Keywords: | "Caproates/metabolism Caprylates/metabolism Catalytic Domain Fatty Acid Synthases/*genetics/metabolism Fatty Acids, Volatile/*biosynthesis Genetic Vectors/chemistry/metabolism Humans Industrial Microbiology Models, Molecular Mutagenesis, Site-Directed Muta;" |
| Notes: | "MedlineGajewski, Jan Pavlovic, Renata Fischer, Manuel Boles, Eckhard Grininger, Martin eng Research Support, Non-U.S. Gov't England 2017/03/11 Nat Commun. 2017 Mar 10; 8:14650. doi: 10.1038/ncomms14650" |
