| Title: | Fast and solvent-free quantitation of boar taint odorants in pig fat by stable isotope dilution analysis-dynamic headspace-thermal desorption-gas chromatography/time-of-flight mass spectrometry |
| Author(s): | Fischer J; Haas T; Leppert J; Lammers PS; Horner G; Wust M; Boeker P; |
| Address: | "University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany; ELFI Analytik GbR, Massenhausener Strasse 18a, D-85375 Neufahrn, Germany. University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany. Five Technologies GmbH, Frauenstrasse 22, 80469 Munich, Germany. University of Bonn, Department of Nutrition and Food Sciences, Bioanalytics, Endenicher Allee 11-13, D-53115 Bonn, Germany. University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany. Electronic address: boeker@uni-bonn.de" |
| DOI: | 10.1016/j.foodchem.2014.02.113 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Linking) |
| Abstract: | "Boar taint is a specific off-odour of boar meat products, known to be caused by at least three unpleasant odorants, with very low odour thresholds. Androstenone is a boar pheromone produced in the testes, whereas skatole and indole originate from the microbial breakdown of tryptophan in the intestinal tract. A new procedure, applying stable isotope dilution analysis (SIDA) and dynamic headspace-thermal desorption-gas chromatography/time-of-flight mass spectrometry (dynHS-TD-GC/TOFMS) for the simultaneous quantitation of these boar taint compounds in pig fat was elaborated and validated in this paper. The new method is characterised by a simple and solvent-free dynamic headspace sampling. The deuterated compounds d3-androstenone, d3-skatole and d6-indole were used as internal standards to eliminate matrix effects. The method validation performed revealed low limits of detection (LOD) and quantitation (LOQ) with high accuracy and precision, thus confirming the feasibility of the new dynHS-TD-GC/TOFMS approach for routine analysis" |
| Keywords: | Androstenes/analysis Animals Fats/*chemistry Gas Chromatography-Mass Spectrometry/instrumentation/*methods Indicator Dilution Techniques Indoles/analysis Male Mass Spectrometry/methods Meat Products/analysis Odorants/*analysis Skatole/analysis Sus scrofa; |
| Notes: | "MedlineFischer, Jochen Haas, Torsten Leppert, Jan Lammers, Peter Schulze Horner, Gerhard Wust, Matthias Boeker, Peter eng Evaluation Study Research Support, Non-U.S. Gov't England 2014/04/16 Food Chem. 2014 Sep 1; 158:345-50. doi: 10.1016/j.foodchem.2014.02.113. Epub 2014 Feb 28" |
