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J Biol Chem

Title:		Metabolism of alpha-factor by a mating type cells of Saccharomyces cerevisiae
Author(s):		Finkelstein DB; Strausberg S;
Address:
Journal Title:		J Biol Chem
Year:		1979
Volume:		254
Issue:		3
Page Number:		796 - 803
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"When a mating type cells of Saccharomyces cerevisiae are exposed to the mating pheromone alpha-factor in liquid cultures, there is a time-dependent loss of alpha-factor activity from the culture fluid. This loss of biological activity can be directly correlated with the proteolysis of the pheromone by a mating type cells. The metabolism of alpha-factor by a mating type cells may be measured by using either in vitro 125I-labeled or in vivo 35S-labeled pheromone. Addition of chloroquine to growing cultures of a mating type cells at concentrations which cause no detectable alterations in cell growth produces a potentiation of alpha-factor mediated cell cycle arrest. This potentiation of alpha-factor activity is directly correlated with the inhibition of alpha-factor proteolysis. Thus, while proteolytic digestion of alpha-factor appears to be related to the mechanism whereby a mating type cells 'detoxify' alpha-factor and recover from cell cycle arrest, proteolysis of the mating factor is not necessary for alpha-factor mediated cell cycle arrest"
Keywords:		"Chloroquine/pharmacology Genetics, Microbial Kinetics Pepsin A Peptides/*metabolism Saccharomyces cerevisiae/metabolism/*physiology Sulfuric Acids/metabolism;"
Notes:		"MedlineFinkelstein, D B Strausberg, S eng Research Support, U.S. Gov't, Non-P.H.S. 1979/02/10 J Biol Chem. 1979 Feb 10; 254(3):796-803"