| Title: | Ethylene is a local modulator of jasmonate-dependent phenolamide accumulation during Manduca sexta herbivory in Nicotiana attenuata |
| Author(s): | Figon F; Baldwin IT; Gaquerel E; |
| Address: | "Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany. Master BioSciences, ENS de Lyon, UCB Lyon 1, Universite de Lyon, Lyon, France. Institut de Biologie Moleculaire des Plantes du CNRS, Universite de Strasbourg, Strasbourg, France" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Rapid reconfigurations of interconnected phytohormone signalling networks allow plants to tune their physiology to constantly varying ecological conditions. During insect herbivory, most of the induced changes in defence-related leaf metabolites are controlled by jasmonate (JA) signalling, which, in the wild tobacco Nicotiana attenuata, recruits MYB8, a transcription factor controlling the accumulation of phenolic-polyamine conjugates (phenolamides). In this and other plant species, herbivory also locally triggers ethylene (ET) production but the outcome of the JA-ET cross-talk at the level of secondary metabolism regulation has remained only superficially investigated. Here, we analysed local and systemic herbivory-induced changes by mass spectrometry-based metabolomics in leaves of transgenic plants impaired in JA, ET and MYB8 signalling. Parsing deregulations in this factorial data-set identified a network of JA/MYB8-dependent phenolamides for which impairment of ET signalling attenuated their accumulation only in locally damaged leaves. Further experiments revealed that ET, albeit biochemically interrelated to polyamine metabolism via the intermediate S-adenosylmethionine, does not alter the free polyamine levels, but instead significantly modulates phenolamide levels with marginal modulations of transcript levels. The work identifies ET as a local modulator of phenolamide accumulations and provides a metabolomics data-platform with which to mine associations among herbivory-induced signalling and specialized metabolites in N. attenuata" |
| Keywords: | "Abscisic Acid/metabolism Amides/metabolism Animals Chromatography, High Pressure Liquid Cyclopentanes/*metabolism Ethylenes/*metabolism *Manduca Oxylipins/*metabolism *Plant Defense Against Herbivory Plant Growth Regulators/*metabolism/physiology Plant Le;" |
| Notes: | "MedlineFigon, Florent Baldwin, Ian T Gaquerel, Emmanuel eng Research Support, Non-U.S. Gov't 2020/11/21 Plant Cell Environ. 2021 Mar; 44(3):964-981. doi: 10.1111/pce.13955. Epub 2020 Dec 15" |
