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Plant Mol Biol

Title:		An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange and lemon
Author(s):		Faddetta T; Abbate L; Renzone G; Palumbo Piccionello A; Maggio A; Oddo E; Scaloni A; Puglia AM; Gallo G; Carimi F; Fatta Del Bosco S; Mercati F;
Address:		"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Palermo, Italy. Advanced Technologies Network (ATeN) Center, University of Palermo, Palermo, Italy. Institute of Biosciences and Bioresources (IBBR), National Research Council, Palermo, Italy. Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, Naples, Italy. Institute of Biosciences and Bioresources (IBBR), National Research Council, Palermo, Italy. francesco.mercati@ibbr.cnr.it"
Journal Title:		Plant Mol Biol
Year:		2018
Volume:		20181019
Issue:		4-May
Page Number:		407 - 425
DOI:		10.1007/s11103-018-0787-9
ISSN/ISBN:		1573-5028 (Electronic) 0167-4412 (Linking)
Abstract:		"Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded traits into cultivated varieties. In these cases, the plant coordinated array of physiological, biochemical, and molecular functions remains the result of integration among different signals, which derive from the compartmentalized genomes of nucleus, plastids and mitochondria. To dissect the effects of genome rearrangement into cybrids, a multidisciplinary study was conducted on a diploid cybrid (C2N), resulting from a breeding program aimed to improve interesting agronomical traits for lemon, the parental cultivars 'Valencia' sweet orange (V) and 'femminello' lemon (F), and the corresponding somatic allotetraploid hybrid (V + F). In particular, a differential proteomic analysis, based on 2D-DIGE and MS procedures, was carried out on leaf proteomes of C2N, V, F and V + F, using the C2N proteome as pivotal condition. This investigation revealed differentially represented protein patterns that can be associated with genome rearrangement and cell compartment interplay. Interestingly, most of the up-regulated proteins in the cybrid are involved in crucial biological processes such as photosynthesis, energy production and stress tolerance response. The cybrid differential proteome pattern was concomitant with a general increase of leaf gas exchange and content of volatile organic compounds, highlighting a stimulation of specific pathways that can be related to observed plant performances. Our results contribute to a better understanding how the alloplasmic condition might lead to a substantial improvement in plant breeding, opening new opportunities to develop varieties more adaptable to a wide range of conditions"
Keywords:		"Cell Nucleus/metabolism/*physiology Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Citrus/*genetics/metabolism Citrus sinensis/*genetics/metabolism Cytoplasm/metabolism/*physiology *Diploidy Disaccharides Electrophoresis, Gel, Two-Dime;"
Notes:		"MedlineFaddetta, Teresa Abbate, Loredana Renzone, Giovanni Palumbo Piccionello, Antonio Maggio, Antonella Oddo, Elisabetta Scaloni, Andrea Puglia, Anna Maria Gallo, Giuseppe Carimi, Francesco Fatta Del Bosco, Sergio Mercati, Francesco eng Netherlands 2018/10/21 Plant Mol Biol. 2018 Nov; 98(4-5):407-425. doi: 10.1007/s11103-018-0787-9. Epub 2018 Oct 19"