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Acta Pharm

Title:		Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)
Author(s):		Abdel-Lateef E; Mahmoud F; Hammam O; El-Ahwany E; El-Wakil E; Kandil S; Abu Taleb H; El-Sayed M; Hassenein H;
Address:
Journal Title:		Acta Pharm
Year:		2016
Volume:		66
Issue:		3
Page Number:		387 - 398
DOI:		10.1515/acph-2016-0028
ISSN/ISBN:		1846-9558 (Electronic) 1330-0075 (Linking)
Abstract:		"The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), beta-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 +/- 2.87 mug mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 +/- 5.25 mug mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 mug g-1 dried methanol extract) in C. longa rhizomes"
Keywords:		"Antineoplastic Agents, Phytogenic/analysis/*chemistry/isolation & purification/pharmacology Apoptosis/*drug effects Carcinoma, Hepatocellular/*drug therapy/pathology Cell Proliferation/drug effects Cell Survival/drug effects Chromatography, High Pressure;"
Notes:		"MedlineAbdel-Lateef, Ezzat Mahmoud, Faten Hammam, Olfat El-Ahwany, Eman El-Wakil, Eman Kandil, Sherihan Abu Taleb, Hoda El-Sayed, Mortada Hassenein, Hanaa eng Comparative Study Poland 2016/07/08 Acta Pharm. 2016 Sep 1; 66(3):387-98. doi: 10.1515/acph-2016-0028"