« Previous AbstractIdentification of a queen primer pheromone in higher termites    Next AbstractMenthol addition to cigarettes using breakable capsules in the filter. Impact on the mainstream smoke yields of the health Canada list constituents »

Anal Biochem

Title:		Development of an analytical method for the determination of sterol compounds in boars' saliva
Author(s):		Dolezal P; Pokorna K; Citek J; Stupka R;
Address:		"Department of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Praha - Suchdol, Czech Republic; Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Praha - Suchdol, Czech Republic. Electronic address: dolezal1@af.czu.cz. Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Praha - Suchdol, Czech Republic"
Journal Title:		Anal Biochem
Year:		2022
Volume:		20220930
Issue:
Page Number:		114932 -
DOI:		10.1016/j.ab.2022.114932
ISSN/ISBN:		1096-0309 (Electronic) 0003-2697 (Linking)
Abstract:		"'Boar taint' compounds influence the sexual behavioral responses of sows and stimulate their reproduction. This paper reports a fast, easier, and a non-invasive analytical method for the analysis of three 'boar taint' compounds in boar' saliva samples: androstenone, androsten-3alpha-ol, and androsten-3beta-ol. This method was developed and validated based on solid-phase extraction (SPE) and multidimensional gas chromatography-mass spectrometry (MDGC-MS). All the compounds were detected without derivatization. This method affords good reproducibility (4%-8%), accuracy (80%-105%), precision (5.5%-9.1%), linearity (R(2) = 0.98-0.99), and lower limits of quantitation (LLOQ) (0.1-0.2 mug/L). Although the presence of these compounds in saliva has been known for a long time, no simple and easy analytical method has been developed"
Keywords:		Swine Animals Male Female *Skatole/analysis *Sterols Saliva/chemistry Reproducibility of Results Indoles/analysis Androstenone Boar taint Mdgc-ms Pheromones SPE-Extraction Sterol compounds;
Notes:		"MedlineDolezal, Petr Pokorna, Kamila Citek, Jaroslav Stupka, Roman eng Research Support, Non-U.S. Gov't 2022/10/04 Anal Biochem. 2022 Dec 1; 658:114932. doi: 10.1016/j.ab.2022.114932. Epub 2022 Sep 30"