| Title: | Exhaled Volatile Organic Compounds during Inflammation Induced by TNF-alpha in Ventilated Rats |
| Author(s): | Albrecht FW; Maurer F; Muller-Wirtz LM; Schwaiblmair MH; Huppe T; Wolf B; Sessler DI; Volk T; Kreuer S; Fink T; |
| Address: | "CBR-Center of Breath Research, Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center and Saarland University Faculty of Medicine, 66421 Homburg/Saar, Germany. Department of Outcomes Research, Anesthesiology Institute, Cleveland Clinic, Cleveland, OH 44195, USA" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "Systemic inflammation alters the composition of exhaled breath, possibly helping clinicians diagnose conditions such as sepsis. We therefore evaluated changes in exhaled breath of rats given tumor necrosis factor-alpha (TNF-alpha). Thirty male Sprague-Dawley rats were randomly assigned to three groups (n = 10 each) with intravenous injections of normal saline (control), 200 microg.kg(-1) bodyweight TNF-alpha (TNF-alpha-200), or 600 microg.kg(-1) bodyweight TNF-alpha (TNF-alpha-600), and were observed for 24 h or until death. Animals were ventilated with highly-purified synthetic air to analyze exhaled air by multicapillary column-ion mobility spectrometry. Volatile organic compounds (VOCs) were identified from a database. We recorded blood pressure and cardiac output, along with cytokine plasma concentrations. Control rats survived the 24 h observation period, whereas mean survival time decreased to 22 h for TNF-alpha-200 and 23 h for TNF-alpha-600 rats. Mean arterial pressure decreased in TNF-alpha groups, whereas IL-6 increased, consistent with mild to moderate inflammation. Hundreds of VOCs were detected in exhalome. P-cymol increased by a factor-of-two 4 h after injection of TNF-alpha-600 compared to the control and TNF-alpha-200. We found that 1-butanol and 1-pentanol increased in both TNF-alpha groups after 20 h compared to the control. As breath analysis distinguishes between two doses of TNF-alpha and none, we conclude that it might help clinicians identify systemic inflammation" |
| Keywords: | Mcc-ims TNF-alpha Voc anesthesia breath analysis inflammation multicapillary-column ion mobility spectrometry; |
| Notes: | "PubMed-not-MEDLINEAlbrecht, Frederic W Maurer, Felix Muller-Wirtz, Lukas M Schwaiblmair, Michaela H Huppe, Tobias Wolf, Beate Sessler, Daniel I Volk, Thomas Kreuer, Sascha Fink, Tobias eng Switzerland 2020/06/19 Metabolites. 2020 Jun 15; 10(6):245. doi: 10.3390/metabo10060245" |
