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Mol Cell Biol

Title:		Yeast L double-stranded ribonucleic acid is synthesized during the G1 phase but not the S phase of the cell cycle
Author(s):		Zakian VA; Wagner DW; Fangman WL;
Address:		"Hutchinson Cancer Research Center, Division of Genetics, Seattle, Washington 98104, USA"
Journal Title:		Mol Cell Biol
Year:		1981
Volume:		1
Issue:		8
Page Number:		673 - 679
DOI:		10.1128/mcb.1.8.673-679.1981
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The cytoplasm of Saccharomyces cerevisiae contains two major classes of protein-encapsulated double-stranded ribonucleic acids (dsRNA's), L and M. Replication of L and M dsRNA's was examined in cells arrested in the G1 phase by either alpha-factor, a yeast mating pheromone, or the restrictive temperature for a cell cycle mutant (cdc7). [3H]uracil was added during the arrest periods to cells prelabeled with [14C]uracil, and replication was monitored by determining the ratio of 3H/14C for purified dsRNA's. Like mitochondrial deoxyribonucleic acid, both L and M dsRNA's were synthesized in the G1 arrested cells. The replication of L dsRNA was also examined during the S phase, using cells synchronized in two different ways. Cells containing the cdc7 mutation, treated sequentially with alpha-factor and then the restrictive temperature, enter a synchronous S phase when transferred to permissive temperature. When cells entered the S phase, synthesis of L dsRNA ceased, and little or no synthesis was detected throughout the S phase. Synthesis of L dsRNA was also observed in G1 phase cells isolated from asynchronous cultures by velocity centrifugation. Again, synthesis ceased when cells entered the S phase. These results indicate that L dsRNA replication is under cell cycle control. The control differs from that of mitochondrial deoxyribonucleic acid, which replicates in all phases of the cell cycle, and from that of 2-micron DNA, a multiple-copy plasmid whose replication is confined to the S phase"
Keywords:		"*G1 Phase RNA, Double-Stranded/*biosynthesis RNA, Fungal/*biosynthesis *S Phase Saccharomyces cerevisiae/cytology/*genetics;"
Notes:		"MedlineZakian, V A Wagner, D W Fangman, W L eng GM18926/GM/NIGMS NIH HHS/ GM26938/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1981/08/01 Mol Cell Biol. 1981 Aug; 1(8):673-9. doi: 10.1128/mcb.1.8.673-679.1981"