| Title: | Associated Bacteria Affect Sexual Reproduction by Altering Gene Expression and Metabolic Processes in a Biofilm Inhabiting Diatom |
| Author(s): | Cirri E; De Decker S; Bilcke G; Werner M; Osuna-Cruz CM; De Veylder L; Vandepoele K; Werz O; Vyverman W; Pohnert G; |
| Address: | "Institute of Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, Jena, Germany. Protistology and Aquatic Ecology, Department of Biology, Ghent University, Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. VIB Center for Plant Systems Biology, Ghent, Belgium. Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, Jena, Germany" |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Diatoms are unicellular algae with a fundamental role in global biogeochemical cycles as major primary producers at the base of aquatic food webs. In recent years, chemical communication between diatoms and associated bacteria has emerged as a key factor in diatom ecology, spurred by conceptual and technological advancements to study the mechanisms underlying these interactions. Here, we use a combination of physiological, transcriptomic, and metabolomic approaches to study the influence of naturally co-existing bacteria, Maribacter sp. and Roseovarius sp., on the sexual reproduction of the biofilm inhabiting marine pennate diatom Seminavis robusta. While Maribacter sp. severely reduces the reproductive success of S. robusta cultures, Roseovarius sp. slightly enhances it. Contrary to our expectation, we demonstrate that the effect of the bacterial exudates is not caused by altered cell-cycle regulation prior to the switch to meiosis. Instead, Maribacter sp. exudates cause a reduced production of diproline, the sexual attraction pheromone of S. robusta. Transcriptomic analyses show that this is likely an indirect consequence of altered intracellular metabolic fluxes in the diatom, especially those related to amino acid biosynthesis, oxidative stress response, and biosynthesis of defense molecules. This study provides the first insights into the influence of bacteria on diatom sexual reproduction and adds a new dimension to the complexity of a still understudied phenomenon in natural diatom populations" |
| Keywords: | bacterial exudates cross-kingdom interactions diatoms metabolomics pheromones transcriptomics; |
| Notes: | "PubMed-not-MEDLINECirri, Emilio De Decker, Sam Bilcke, Gust Werner, Markus Osuna-Cruz, Cristina Maria De Veylder, Lieven Vandepoele, Klaas Werz, Oliver Vyverman, Wim Pohnert, Georg eng Switzerland 2019/08/21 Front Microbiol. 2019 Aug 2; 10:1790. doi: 10.3389/fmicb.2019.01790. eCollection 2019" |
