| Title: | Silencing a Simple Extracellular Leucine-Rich Repeat Gene OsI-BAK1 Enhances the Resistance of Rice to Brown Planthopper Nilaparvata lugens |
| Author(s): | Ye M; Kuai P; Chen S; Lin N; Ye M; Hu L; Lou Y; |
| Address: | "State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Hainan Institute, Zhejiang University, Sanya 572025, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins-the simple eLRR proteins-in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein OsI-BAK1 in rice localizes to the plasma membrane. Its expression was induced by mechanical wounding, the infestation of gravid females of brown planthopper (BPH) Nilaparvata lugens or white-backed planthopper Sogatella furcifera and treatment with methyl jasmonate or abscisic acid. Silencing OsI-BAK1 (ir-ibak1) in rice enhanced the BPH-induced transcript levels of three defense-related WRKY genes (OsWRKY24, OsWRKY53 and OsWRKY70) but decreased the induced levels of ethylene. Bioassays revealed that the hatching rate was significantly lower in BPH eggs laid on ir-ibak1 plants than wild-type (WT) plants; moreover, gravid BPH females preferred to oviposit on WT plants over ir-ibak1 plants. The exogenous application of ethephon on ir-ibak1 plants eliminated the BPH oviposition preference between WT and ir-ibak1 plants but had no effect on the hatching rate of BPH eggs. These findings suggest that OsI-BAK1 acts as a negative modulator of defense responses in rice to BPH and that BPH might exploit this modulator for its own benefit" |
| Keywords: | "Abscisic Acid/pharmacology Acetates/pharmacology Animals Cyclopentanes/pharmacology Ethylenes/metabolism Female Gene Expression Regulation, Plant/drug effects Gene Silencing Hemiptera/*physiology Leucine/*chemistry Organophosphorus Compounds/pharmacology;" |
| Notes: | "MedlineYe, Miaofen Kuai, Peng Chen, Shuting Lin, Na Ye, Meng Hu, Lingfei Lou, Yonggen eng 31930091/National Natural Science Foundation of China/ Switzerland 2021/11/28 Int J Mol Sci. 2021 Nov 10; 22(22):12182. doi: 10.3390/ijms222212182" |
