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Cells Tissues Organs

Title:		Glycoconjugate-specific developmental changes in the horse vomeronasal organ
Author(s):		Chun J; Kang T; Seo JP; Jeong H; Kim M; Kim BS; Ahn M; Kim J; Shin T;
Address:
Journal Title:		Cells Tissues Organs
Year:		2023
Volume:		20230104
Issue:
Page Number:		-
DOI:		10.1159/000528883
ISSN/ISBN:		1422-6421 (Electronic) 1422-6405 (Linking)
Abstract:		"Vomeronasal organ (VNO) is a tubular pheromone sensing organ in which the lumen is covered with sensory and non-sensory epithelia. This study used immunohistochemistry and lectin histochemistry techniques to evaluate developmental changes, specifically of the glycoconjugate profile, in the horse VNO epithelium. Immunostaining analysis revealed PGP9.5 expression in some vomeronasal non-sensory epithelium (VNSE) cells and in the vomeronasal receptor cells of the vomeronasal sensory epithelium (VSE) in fetuses, young foals, and adult horses. OMP expression was exclusively localized in receptor cells of the VSE in fetuses, young foals, and adult horses and absent in VNSE. To identify the glycoconjugate type, lectin histochemistry was performed using 21 lectins. Semi-quantitative analysis revealed that the intensities of glycoconjugates labeled with WGA, DSL, LEL, and RCA120 were significantly higher in adult horse VSE than those in foal VSE, whereas the intensities of glycoconjugates labeled with LCA and PSA were significantly lower in adult horse VSE. The intensities of glycoconjugates labeled with s-WGA, WGA, BSL-II, DSL, LEL, STL, ConA, LCA, PSA, DBA, SBA, SJA, RCA120, jacalin, and ECL were significantly higher in adult horse VNSE than those in foal VNSE, whereas the intensity of glycoconjugates labeled with UEA-I was lower in adult horse VNSE. Histochemical analysis of each lectin revealed that various glycoconjugates in the VSE were present in the receptor, supporting, and basal cells of foal and adult horses. A similar pattern of lectin histochemistry was also observed in the VNSE of foal and adult horses. In conclusion, these results suggest that there are increase in the level of N-acetylglucosamine (labeled by WGA, DSL, LEL) and galactose (labeled by RCA120) in horse VSE during postnatal development, implying that they may influence the function of VNO in adult horses"
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Notes:		"PublisherChun, Jiyoon Kang, Taeyoung Seo, Jong-Pil Jeong, Hyohoon Kim, Minhan Kim, Byung Sun Ahn, Meejung Kim, Jeongtae Shin, Taekyun eng Switzerland 2023/01/05 Cells Tissues Organs. 2023 Jan 4. doi: 10.1159/000528883"