| Title: | "STE16, a new gene required for pheromone production by a cells of Saccharomyces cerevisiae" |
| DOI: | 10.1093/genetics/115.3.441 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "Genes required for mating by a and alpha cells of Saccharomyces cerevisiae (STE, 'sterile,' genes) encode products such as peptide pheromones, pheromone receptors, and proteins responsible for pheromone processing. a-specific STE genes are those required for mating by a cells but not by alpha cells. To identify new a-specific STE genes, we have employed a novel strategy that enabled us to determine if a ste mutant defective in mating as a is also defective in mating as alpha without the need to do crosses. This technique involved a strain (K12-14b) of genotype mata1 HML alpha HMR alpha sir3ts, which mates as a at 25 degrees and as alpha at 34 degrees. We screened over 40,000 mutagenized colonies derived from K12-14b and obtained 28 a-specific ste mutants. These strains contained mutations in three known a-specific genes--STE2, STE6 and STE14--and in a new gene, STE16. ste16 mutants are defective in the production of the pheromone, a-factor, and exhibit slow growth. Based on the distribution of a-specific ste mutants described here, we infer that we have identified most if not all nonessential genes that can give rise to a-specific mating defects" |
| Keywords: | "Crosses, Genetic *Genes *Genes, Fungal Genetic Complementation Test Mating Factor Mutation Peptides/*genetics Phenotype Pheromones/*genetics Saccharomyces cerevisiae/*genetics;" |
| Notes: | "MedlineWilson, K L Herskowitz, I eng AI18738/AI/NIAID NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1987/03/01 Genetics. 1987 Mar; 115(3):441-9. doi: 10.1093/genetics/115.3.441" |
