| Title: | Selectivity and ligand-based molecular modeling of an odorant-binding protein from the leaf beetle Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) in relation to habitat-related volatiles |
| Author(s): | Wang Y; Jin Y; Chen Q; Wen M; Zhao H; Duan H; Ren B; |
| Address: | "Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, Jilin, China. Key Laboratory of Vegetation Ecology, MOE, Northeast Normal University, Changchun, China. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, China. hxduan@cau.edu.cn. Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, Jilin, China. bzren@163.com. Key Laboratory of Vegetation Ecology, MOE, Northeast Normal University, Changchun, China. bzren@163.com" |
| DOI: | 10.1038/s41598-017-15538-8 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "In this study, the most abundant and antenna-specific odorant-binding protein (OBP) of the elm pest A. quadriimpressum, AquaOBP4, was expressed and purified. The selectivity of AquaOBP4 was investigated by screening against a panel of 40 habitat-relevant compounds. Based on the obtained results, a homologous model of AquaOBP4 was established. This model indicated that AquaOBP4 is highly homologous to DmelOBP LUSH and includes two main binding sites. A docking analysis showed that four of five active ligands bound at Site 1, whereas the other ligand was situated at Site 2. Furthermore, new ligands were docked in the model, and the results of fluorescence-based binding assays of these compounds were highly consistent with the binding conformation and binding affinity predicted by our model. Additionally, three binding odorants derived from elm leaves elicited a strong electroantennogram response and exerted a significant attractive effect on adult A. quadriimpressum. All of the results showed that AquaOBP4 is likely linked to the foraging behavior of A. quadriimpressum. This study provides a new reliable tool for future large-scale compound screenings and revealed several functional chemicals that might aid the development of a better pest management approach for A. quadriimpressum" |
| Keywords: | "Animals Coleoptera/*chemistry Insect Proteins/*chemistry *Molecular Docking Simulation Receptors, Odorant/*chemistry Volatile Organic Compounds/*chemistry;" |
| Notes: | "MedlineWang, Yinliang Jin, Yincan Chen, Qi Wen, Ming Zhao, Hanbo Duan, Hongxia Ren, Bingzhong eng Research Support, Non-U.S. Gov't England 2017/11/15 Sci Rep. 2017 Nov 13; 7(1):15374. doi: 10.1038/s41598-017-15538-8" |
