« Previous Abstract"Thermogenesis, flowering and the association with variation in floral odour attractants in Magnolia sprengeri (Magnoliaceae)"    Next AbstractSwellable Array Strategy Based on Designed Flexible Double Hypercross-linked Polymers for Synergistic Adsorption of Toluene and Formaldehyde »

Chemosphere

Title:		Preliminary analysis of allelochemicals produced by the diatom Phaeodactylum tricornutum
Author(s):		Wang R; Wang J; Xue Q; Tan L; Cai J; Wang H;
Address:		"Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: jtwang@ouc.edu.cn"
Journal Title:		Chemosphere
Year:		2016
Volume:		20160930
Issue:
Page Number:		298 - 303
DOI:		10.1016/j.chemosphere.2016.09.005
ISSN/ISBN:		1879-1298 (Electronic) 0045-6535 (Linking)
Abstract:		"Marine diatom Phaeodactylum tricornutum is known to exude allelochemicals with negative effects on Heterosigma akashiwo according to our previous study, while the information about the allelochemical compounds remains unknown. The present study dealt with isolation and analysis of the active substances released by P. tricornutum into the culture medium. Filtrate of P. tricornutum was extracted using ethyl acetate and chloroform respectively. The anti-algal fractions were isolated using high performance liquid chromatography (HPLC) and screened using activity-guided fraction methods. Results demonstrated that fraction ?af and ?a- showed significant allelopathic effect on H. akashiwo growth. Then the anti-algal activity fractions were analyzed preliminary using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography-electrospray time-of-flight mass spectrometry (HPLC-ESI-TOF-MS). An active compound was derived from fraction ?a- with the molecular weight of 578 and possible molecular formula of C(30)H(38)N(6)O(6), which was speculated to be TYR-PRO-PHE-PRO-GLY-NH(2). a kind of glycinamides"
Keywords:		"Amino Acid Sequence Chromatography, High Pressure Liquid Diatoms/chemistry/*metabolism Dinoflagellida/*drug effects Endorphins/*analysis/metabolism Gas Chromatography-Mass Spectrometry Molecular Weight Peptide Fragments/*analysis/metabolism Pheromones/*an;"
Notes:		"MedlineWang, Rui Wang, Jiangtao Xue, Qiaona Tan, Liju Cai, Jun Wang, Hongyao eng England 2016/09/24 Chemosphere. 2016 Dec; 165:298-303. doi: 10.1016/j.chemosphere.2016.09.005. Epub 2016 Sep 30"