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Methods Mol Biol

Title:		Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes
Author(s):		Spitzer-Rimon B; Cna'ani A; Vainstein A;
Address:		"The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		975
Issue:
Page Number:		139 - 148
DOI:		10.1007/978-1-62703-278-0_10
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting"
Keywords:		"Agrobacterium tumefaciens/virology Base Sequence Flowers/*genetics/metabolism/virology Gene Expression Gene Knockdown Techniques/*methods Genes, Plant Genetic Vectors Petunia/*genetics/metabolism/virology Plant Extracts/chemistry/isolation & purification/;"
Notes:		"MedlineSpitzer-Rimon, Ben Cna'ani, Alon Vainstein, Alexander eng Research Support, Non-U.S. Gov't 2013/02/07 Methods Mol Biol. 2013; 975:139-48. doi: 10.1007/978-1-62703-278-0_10"