| Title: | Sniffing out resistance - Rapid identification of urinary tract infection-causing bacteria and their antibiotic susceptibility using volatile metabolite profiles |
| Author(s): | Smart A; de Lacy Costello B; White P; Avison M; Batty C; Turner C; Persad R; Ratcliffe N; |
| Address: | "Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom. Electronic address: Amy.smart@uwe.ac.uk. Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom. Electronic address: Ben.delacycostello@uwe.ac.uk. Department of Engineering, Design and Mathematics, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom. Electronic address: Paul.white@uwe.ac.uk. School of Cellular and Molecular Medicine, University of Bristol, University Walk, Bristol, BS8 1TD, United Kingdom. Electronic address: Matthewb.Avison@bristol.ac.uk. Faculty of Science, Technology, Engineering & Mathematics, The Open University, Milton Keynes, MK7 6BJ, United Kingdom. Electronic address: Claire.batty@open.ac.uk. Faculty of Science, Technology, Engineering & Mathematics, The Open University, Milton Keynes, MK7 6BJ, United Kingdom. Electronic address: Claire.turner@open.ac.uk. Bristol Urological Institute, Southmead Hospital, Bristol, BS10 5BN, United Kingdom. Electronic address: Rajpersad@bristolurology.com. Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom. Electronic address: Norman.ratcliffe@uwe.ac.uk" |
| DOI: | 10.1016/j.jpba.2019.01.044 |
| ISSN/ISBN: | 1873-264X (Electronic) 0731-7085 (Linking) |
| Abstract: | "Antibiotic resistance is set to be an unprecedented threat to modern medicine. 'Sniffing' bacteria potentially offers a rapid way to determine susceptibility. A successful proof-of-principle study is described, using thermal desorption-gas chromatography-mass spectrometry (TDGCMS) to 'smell' cephalexin and ciprofloxacin resistant and sensitive Urinary Tract Infection (UTI)-causing bacteria. 578 peaks at unique retention times were detected from 86 chromatograms of 18 bacterial isolates (E. coli, K. pneumoniae and P. aeruginosa). The isolates were grown with and without the presence of antibiotic. Chi-square analysis found 9 compounds that differed significantly between cephalexin sensitive and resistant isolates, and 22 compounds that differed significantly between ciprofloxacin sensitive and resistant isolates, at p |
| Keywords: | "Anti-Bacterial Agents/*pharmacology Bacterial Infections/*microbiology Cephalexin/pharmacology Ciprofloxacin/pharmacology Drug Resistance, Bacterial/*drug effects Escherichia coli/drug effects/isolation & purification/metabolism Humans Klebsiella pneumoni;" |
| Notes: | "MedlineSmart, Amy de Lacy Costello, Ben White, Paul Avison, Matthew Batty, Claire Turner, Claire Persad, Raj Ratcliffe, Norman eng England 2019/02/12 J Pharm Biomed Anal. 2019 Apr 15; 167:59-65. doi: 10.1016/j.jpba.2019.01.044. Epub 2019 Jan 31" |
