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Metabolites

Title:		Real Time Breath Analysis Using Portable Gas Chromatography for Adult Asthma Phenotypes
Author(s):		Sharma R; Zang W; Zhou M; Schafer N; Begley LA; Huang YJ; Fan X;
Address:		"Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA"
Journal Title:		Metabolites
Year:		2021
Volume:		20210423
Issue:		5
Page Number:		-
DOI:		10.3390/metabo11050265
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"Asthma is heterogeneous but accessible biomarkers to distinguish relevant phenotypes remain lacking, particularly in non-Type 2 (T2)-high asthma. Moreover, common clinical characteristics in both T2-high and T2-low asthma (e.g., atopy, obesity, inhaled steroid use) may confound interpretation of putative biomarkers and of underlying biology. This study aimed to identify volatile organic compounds (VOCs) in exhaled breath that distinguish not only asthmatic and non-asthmatic subjects, but also atopic non-asthmatic controls and also by variables that reflect clinical differences among asthmatic adults. A total of 73 participants (30 asthma, eight atopic non-asthma, and 35 non-asthma/non-atopic subjects) were recruited for this pilot study. A total of 79 breath samples were analyzed in real-time using an automated portable gas chromatography (GC) device developed in-house. GC-mass spectrometry was also used to identify the VOCs in breath. Machine learning, linear discriminant analysis, and principal component analysis were used to identify the biomarkers. Our results show that the portable GC was able to complete breath analysis in 30 min. A set of nine biomarkers distinguished asthma and non-asthma/non-atopic subjects, while sets of two and of four biomarkers, respectively, further distinguished asthmatic from atopic controls, and between atopic and non-atopic controls. Additional unique biomarkers were identified that discriminate subjects by blood eosinophil levels, obese status, inhaled corticosteroid treatment, and also acute upper respiratory illnesses within asthmatic groups. Our work demonstrates that breath VOC profiling can be a clinically accessible tool for asthma diagnosis and phenotyping. A portable GC system is a viable option for rapid assessment in asthma"
Keywords:		asthma exhaled breath portable gas chromatography precision medicine;
Notes:		"PubMed-not-MEDLINESharma, Ruchi Zang, Wenzhe Zhou, Menglian Schafer, Nicole Begley, Lesa A Huang, Yvonne J Fan, Xudong eng R01 AI129958/AI/NIAID NIH HHS/ R01AI129958/NH/NIH HHS/ Switzerland 2021/05/01 Metabolites. 2021 Apr 23; 11(5):265. doi: 10.3390/metabo11050265"