« Previous AbstractProton transfer reaction ion trap mass spectrometer    Next AbstractPheromone receptors and their putative ligands: possible role in humans »

Chembiochem

Title:		Regulation of Isoprenoid Pheromone Biosynthesis in Bumblebee Males
Author(s):		Prchalova D; Bucek A; Brabcova J; Zacek P; Kindl J; Valterova I; Pichova I;
Address:		"Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i. Flemingovo n. 2, 166 10, Prague 6, Czech Republic. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i. Flemingovo n. 2, 166 10, Prague 6, Czech Republic. iva.pichova@uochb.cas.cz"
Journal Title:		Chembiochem
Year:		2016
Volume:		20151222
Issue:		3
Page Number:		260 - 267
DOI:		10.1002/cbic.201500415
ISSN/ISBN:		1439-7633 (Electronic) 1439-4227 (Linking)
Abstract:		"Males of the closely related species Bombus terrestris and Bombus lucorum attract conspecific females by completely different marking pheromones. MP of B. terrestris and B. lucorum pheromones contain mainly isoprenoid (ISP) compounds and fatty acid derivatives, respectively. Here, we studied the regulation of ISP biosynthesis in both bumblebees. RNA-seq and qRT-PCR analyses indicated that acetoacetyl-CoA thiolase (AACT), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), and farnesyl diphosphate synthase (FPPS) transcripts are abundant in the B. terrestris labial gland. Maximal abundance of these transcripts correlated well with AACT enzymatic activity detected in the LG extracts. In contrast, transcript abundances of AACT, HMGR, and FPPS in B. lucorum were low, and AACT activity was not detected in LGs. These results suggest that transcriptional regulation plays a key role in the control of ISP biosynthetic gene expression and ISP pheromone biosynthesis in bumblebee males"
Keywords:		"Animals Bees/chemistry/genetics/*metabolism Contig Mapping Female Gene Expression Regulation Insect Proteins/genetics/metabolism Male Pheromones/*biosynthesis/chemistry RNA/chemistry/isolation & purification/metabolism Sequence Analysis, RNA Terpenes/chem;"
Notes:		"MedlinePrchalova, Darina Bucek, Ales Brabcova, Jana Zacek, Petr Kindl, Jiri Valterova, Irena Pichova, Iva eng Research Support, Non-U.S. Gov't Germany 2015/12/04 Chembiochem. 2016 Feb 2; 17(3):260-7. doi: 10.1002/cbic.201500415. Epub 2015 Dec 22"