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Cold Spring Harb Protoc

Title:		Synchronization of S Phase in Schizosaccharomyces pombe Cells by Transient Exposure to M-Factor Pheromone
Author(s):		Nielsen O;
Address:		"Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark"
Journal Title:		Cold Spring Harb Protoc
Year:		2016
Volume:		20160901
Issue:		9
Page Number:		-
DOI:		10.1101/pdb.prot091272
ISSN/ISBN:		1559-6095 (Electronic) 1559-6095 (Linking)
Abstract:		"A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase"
Keywords:		*DNA Replication Pheromones/*metabolism S Phase/*drug effects Schizosaccharomyces/*drug effects/*growth & development/metabolism;
Notes:		"MedlineNielsen, Olaf eng 2016/09/03 Cold Spring Harb Protoc. 2016 Sep 1; 2016(9). doi: 10.1101/pdb.prot091272"