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Arch Insect Biochem Physiol

Title:		Structure and activity of Bombyx PBAN
Author(s):		Nagasawa H; Kuniyoshi H; Arima R; Kawano T; Ando T; Suzuki A;
Address:		"Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan"
Journal Title:		Arch Insect Biochem Physiol
Year:		1994
Volume:		25
Issue:		4
Page Number:		261 - 270
DOI:		10.1002/arch.940250403
ISSN/ISBN:		0739-4462 (Print) 0739-4462 (Linking)
Abstract:		"Two structurally related molecular species of pheromone biosynthesis activating neuropeptides (PBANs), PBAN-I and -II, were isolated from adult heads of the silkworm, Bombyx mori, and characterized. PBAN-I is a carboxyl-terminally amidated 33-residue peptide. Structure-activity relationship studies revealed that 1) its carboxyl-terminal pentapeptide is the smallest size showing activity, 2) the carboxyl-terminal amide is indispensable for activity, and 3) oxidation of three Met residues in PBAN-I to Met(O) (methionine sulfoxide) caused marked enhancement of activity, and the three Met(O) residues contribute equally to the enhancement of activity. Molecular design of PBAN analogs using a carboxyl-terminal hexapeptide showed that modification of the amino-terminal amino group brought about a dramatic increase in activity. This increase was presumed to be mainly due to the increased stability in hemolymph. PBANs share the common carboxyl-terminal sequence, -Phe-Xaa-Pro-Arg-Leu-NH2, with myotropic peptides isolated from locust and cockroach. Examination of cross-activity of these two groups of peptides revealed that PBAN and its analogs exhibited myotropic activity comparable to myotropic peptides, while myotropic peptides showed extremely high pheromonotropic activity. In B. mori, PBAN activates sex pheromone (bombykol) production presumably by promoting the reduction reaction from acyl to alcohol, which is the last step in the biosynthesis of bombykol"
Keywords:		"Amino Acid Sequence Animals Bombyx/drug effects/*metabolism Female Molecular Sequence Data Neuropeptides/chemical synthesis/*chemistry/*pharmacology Palmitic Acid Palmitic Acids/metabolism Peptides, Cyclic/chemical synthesis/chemistry Pheromones/chemical;"
Notes:		"MedlineNagasawa, H Kuniyoshi, H Arima, R Kawano, T Ando, T Suzuki, A eng Comparative Study Research Support, Non-U.S. Gov't Review 1994/01/01 Arch Insect Biochem Physiol. 1994; 25(4):261-70. doi: 10.1002/arch.940250403"