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Comp Biochem Physiol C Toxicol Pharmacol

Title:		Pipa carvalhoi skin secretion profiling: absence of peptides and identification of kynurenic acid as the major constitutive component
Author(s):		Mariano DO; Yamaguchi LF; Jared C; Antoniazzi MM; Sciani JM; Kato MJ; Pimenta DC;
Address:		"Laboratorio de Bioquimica e Biofisica, Instituto Butantan, Av Vital Brasil 1500, Sao Paulo, SP, 05503-900, Brazil. Instituto de Quimica - Universidade de Sao Paulo, Av. Prof. Lineu Prestes, 748, Sala 1115, Bloco 11 terreo, Sao Paulo, SP 05508-000, Brazil. Laboratorio de Biologia Celular, Instituto Butantan, Av Vital Brasil 1500, Sao Paulo, SP 05503-900, Brazil. Laboratorio de Bioquimica e Biofisica, Instituto Butantan, Av Vital Brasil 1500, Sao Paulo, SP, 05503-900, Brazil. Electronic address: daniel.pimenta@butantan.gov.br"
Journal Title:		Comp Biochem Physiol C Toxicol Pharmacol
Year:		2015
Volume:		20140823
Issue:
Page Number:		1 - 6
DOI:		10.1016/j.cbpc.2014.08.001
ISSN/ISBN:		1532-0456 (Print) 1532-0456 (Linking)
Abstract:		"The presence of peptides has been identified in all African pipid genera; nevertheless, little is known about skin secretion of South American frog genus Pipa. Skin secretion from captive and wild Pipa carvalhoi were obtained in the presence or absence of norepinephrine stimulation. The <10 kDa fraction was analyzed by liquid chromatography and mass spectrometry, searching for peptides. Chromatographic profiles show the presence of a major component in this secretion, regardless of the stimulation method (norepinephrine or mechanical stimulation) and the origin of the animal (captivity or wild), as well as in the absence of any stimulus. The general mass distribution profile in P. carvalhoi skin secretion shows numerous components below 800 Da. Moreover, no peptide could be identified, regardless of the chromatographic approach. The major component was purified and identified as kynurenic acid, an L-tryptophan derivative. P. carvalhoi does not secrete peptides as toxins in its skin. In addition, we here report that kynurenic acid is the main component of P. carvalhoi skin secretion. Although no biological activity was associated with kynurenic acid, we propose that this molecule is a pheromone that signals the presence of a co-specific in the shady environment in which this animal lives. In this study we demonstrate the absence of peptidic toxins in the skin secretion of P. carvalhoi, a break of paradigm in the pipid family"
Keywords:		Animals Anura/*metabolism Chemical Phenomena Kynurenic Acid/*metabolism Skin/*metabolism Anura Granular glands Kynurenic acid Pipa carvalhoi Pipidae Xenopus laevis;
Notes:		"MedlineMariano, Douglas Oscar Ceolin Yamaguchi, Lydia Fumiko Jared, Carlos Antoniazzi, Marta Maria Sciani, Juliana Mozer Kato, Massuo Jorge Pimenta, Daniel Carvalho eng Research Support, Non-U.S. Gov't 2014/09/04 Comp Biochem Physiol C Toxicol Pharmacol. 2015 Jan; 167:1-6. doi: 10.1016/j.cbpc.2014.08.001. Epub 2014 Aug 23"