| Title: | Primary culture of antennal cells of Mamestra brassicae: morphology of cell types and evidence for biosynthesis of pheromone-binding proteins in vitro |
| Author(s): | Lucas P; Nagnan-Le Meillour P; |
| Address: | "INRA, Unite de Phytopharmacie et des Mediateurs Chimiques, Route de Saint Cyr, F-78026 Versailles Cedex, France. plucas@versailles.inra.fr" |
| ISSN/ISBN: | 0302-766X (Print) 0302-766X (Linking) |
| Abstract: | "A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytochemically with anti-HRP staining and could be separated into two subtypes, A1 and A2, on the basis of the size of the cell body. Non-neuronal cells could be divided into four types. Type-B cells consisted of large and flat cells with a veil-like cytoplasm. They usually adhered to each other and formed groups of 20 to 50 cells. Type C were large and flat cells, highly variable in size and shape. They closely resembled insect glial cells in culture. Type-D cells grew thick processes, up to 100 microm long. These elongated processes resembled trichoid hairs, and D cells are most probably trichogen cells. Type E were spindle-shaped cells present in low number in the cultures. A high proportion of similar cells in the hemolymph of pupae suggests that E cells are hemocytes. Pheromone-binding proteins were detected using specific antisera in the medium of 3- to 4-week-old cultures" |
| Keywords: | "Animals Blotting, Western Cells, Cultured Insect Proteins/*biosynthesis Moths/chemistry/*cytology Neurons/cytology Pheromones/metabolism;" |
| Notes: | "MedlineLucas, P Nagnan-Le Meillour, P eng Research Support, Non-U.S. Gov't Germany 1997/08/01 Cell Tissue Res. 1997 Aug; 289(2):375-82. doi: 10.1007/s004410050884" |
