| Title: | Reverse chemical ecology guides the screening for Grapholita molesta pheromone synergists |
| Author(s): | Liu J; Zhou T; Li C; Li R; Ye X; Tian Z; |
| Address: | "Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, China. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Pheromone-based management is a leading nonpesticidal strategy among integrated pest management options. Improving the potency of pheromone products by adding synergists would be a practical way to popularize pheromone-based management as well as to reduce pesticide use. RESULTS: Using reverse chemical ecology, synergists for Grapholita molesta sex pheromone were screened. Combined molecular docking and in vitro binding assay led to the determination of four potentially active odorants showing high affinity to G. molesta pheromone binding protein 2 (GmolPBP2). Thereafter, the high affinity between Codlemone and GmolPBP2 was further verified by exploration of GmolPBP2-Codlemone interactions. As the only sex pheromone synergist validated by both laboratory behavioral tests and field trapping, Codlemone was used to optimize commercial sex attractants currently used in G. molesta control. The recommended formulation [(Z)-8-dodecenyl acetate:(E)-8-dodecenyl acetate:Codlemone = 95:4:10] was found to trap about five to six times more G. molesta adults than the commercial sex attractant [(Z)-8-dodecenyl acetate:(E)-8-dodecenyl acetate: (Z)-8-dodecenol = 95:4:1]. CONCLUSION: Codlemone is an excellent pheromone synergist that can be potentially sensed by GmolPBP2, which can remarkably improve the potency of G. molesta sex attractants. It is believed that the introduction of reverse chemical ecology would increase the chance of discovering pheromone synergists, promoting the development of more efficacious pheromone products that can be used in controlling G. molesta and beyond. (c) 2021 Society of Chemical Industry" |
| Keywords: | Animals Molecular Docking Simulation *Moths Pheromones/pharmacology *Sex Attractants/pharmacology Sex Work molecular docking oriental fruit moth pheromone synergist pheromone-based management reverse chemical ecology; |
| Notes: | "MedlineLiu, Jiyuan Zhou, Tong Li, Chaoxia Li, Ruichi Ye, Xuan Tian, Zhen eng Chinese Universities Scientific Fund/ National Natural Science Foundation of China/ Natural Science Foundation of Jiangsu Province/ Sci-Tech Planning Project of Yangling Demonstration Zone/ England 2021/10/19 Pest Manag Sci. 2022 Feb; 78(2):643-652. doi: 10.1002/ps.6674. Epub 2021 Oct 30" |
