| Title: | "Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2" |
| Address: | "Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448" |
| DOI: | 10.1016/0092-8674(90)90503-7 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "alpha factor is a negative growth factor and differentiation factor that induces G1 arrest and increases transcription of mating genes in S. cerevisiae a cells. We have identified a gene, FAR1 (for 'factor arrest'), which is necessary for cell cycle arrest but not for other responses to alpha factor: far1- mutants are insensitive to arrest despite having an intact signal transduction pathway. FAR1 is a nonessential gene whose expression is induced 4- to 5-fold in a cells by alpha factor. The sequence of FAR1 indicates no significant similarities to known proteins. A null mutation in the CLN2 gene, which codes for a G1 cyclin, reverses the effect of a far1 null mutation: far1- cln2- strains arrest in response to alpha factor. We thus propose that FAR1 contributes to cell cycle arrest by inhibiting CLN2. The behavior of far1- cln2- strains indicates that products other than FAR1 are responsible for inhibiting the other G1 cyclins, CLN1 and CLN3" |
| Keywords: | "Amino Acid Sequence Base Sequence Cell Cycle Chromosome Deletion Cloning, Molecular Cyclins/biosynthesis/*genetics *Genes, Fungal Genes, Suppressor Genotype Interphase Mating Factor Molecular Sequence Data Open Reading Frames Peptides/physiology Pheromone;" |
| Notes: | "MedlineChang, F Herskowitz, I eng GM31286/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1990/11/30 Cell. 1990 Nov 30; 63(5):999-1011. doi: 10.1016/0092-8674(90)90503-7" |
