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Cancer Biomark

Title:		Real-time ultrasensitive VUV-PIMS detection of representative endogenous volatile markers in cancers
Author(s):		Li Z; Shu J; Zhang P; Sun W; Yang B; Zhang H;
Address:
Journal Title:		Cancer Biomark
Year:		2016
Volume:		16
Issue:		3
Page Number:		477 - 487
DOI:		10.3233/CBM-160587
ISSN/ISBN:		1875-8592 (Electronic) 1574-0153 (Linking)
Abstract:		"BACKGROUND: Identifying endogenous volatile organic compounds (VOCs) as markers for different cancers currently requires time-consuming procedures and specialized operators. OBJECTIVE: The objective of this study was to develop a rapid and simple method for measuring VOCs at trace levels. METHODS: A simple vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS) was used to detect trace levels of dimethyl trisulfide (DMTS), dimethyl sulfide (DMS), and 2-butanone, which correspond to volatile biomarker candidates present in the exhaled breath of patients with breast, liver, and lung cancers, respectively. The practicality of measuring endogenous VOCs using VUV-PIMS was confirmed by detecting them in cultured cell lines. RESULTS: The abovementioned VOCs were detected with high sensitivity by VUV-PIMS. The limits of detection (LODs) for DMTS, DMS, and 2-butanone were 3.1, 3.9, and 23.2 pptv, respectively, under ambient conditions, which surpass the sensitivity of nearly all other MS-based techniques. Moreover, relatively high concentrations of 2-butanone and DMS were observed in VOCs emitted from the A549 lung cancer cell line and the HepG2 liver cancer cell line, respectively. CONCLUSIONS: Our results show that VUV-PIMS may serve as a reliable method for real-time measurement of endogenous volatile cancer biomarkers"
Keywords:		"A549 Cells Biomarkers, Tumor/*analysis Breast Neoplasms/diagnosis Breath Tests/*methods Butanones/*analysis Cell Line, Tumor Exhalation Female Gas Chromatography-Mass Spectrometry HEK293 Cells Hep G2 Cells Humans Limit of Detection Liver Neoplasms/diagnos;"
Notes:		"MedlineLi, Zhen Shu, Jinian Zhang, Peng Sun, Wanqi Yang, Bo Zhang, Haixu eng Research Support, Non-U.S. Gov't Netherlands 2016/04/12 Cancer Biomark. 2016; 16(3):477-87. doi: 10.3233/CBM-160587"