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Pest Manag Sci

Title:		The regulation of three new members of the cytochrome P450 CYP6 family and their promoters in the cotton aphid Aphis gossypii by plant allelochemicals
Author(s):		Li F; Ma K; Chen X; Zhou JJ; Gao X;
Address:		"Department of Entomology, China Agricultural University, Beijing, P. R. China. Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, UK"
Journal Title:		Pest Manag Sci
Year:		2019
Volume:		20180829
Issue:		1
Page Number:		152 - 159
DOI:		10.1002/ps.5081
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: The expression of P450 genes in insects can be induced by plant allelochemicals. To understand the induction mechanisms, we measured the expression profiles of three P450 genes and their promoter activities under the induction of plant allelochemicals. RESULTS: The inducible expression of CYP6CY19 was the highest among three genes, followed by those of CYP6CY22 and CYP6DA1. The regions from -687 to +586 bp of CYP6DA1, from -666 to +140 bp of CYP6CY19 and from -530 to +218 bp of CYP6CY22 were essential for basal transcriptional activity. The cis-elements for plant allelochemicals induction were identified between -193 and +56 bp of CYP6DA1, between -157 and +140 bp of CYP6CY19 and between -108 and +218 bp of CYP6CY22. These promoter regions were found to contain a potential aryl hydrocarbon receptor element binding site with a conservative sequence motif 5'-C/TAC/ANCA/CA-3'. All these four plant allelochemicals were able to induce the expression of these P450 genes. Tannic acid had a better inductive effect than other three plant allelochemicals. CONCLUSIONS: Our study identified the plant allelochemical responsive cis-elements. This provides further research targets aimed at understanding the regulatory mechanisms of P450 genes expression and their interactions with plant allelochemicals in insect pests. (c) 2018 Society of Chemical Industry"
Keywords:		"Animals Aphids/*drug effects/*genetics/metabolism Cytochrome P450 Family 6/*genetics/metabolism Gossypol/pharmacology Insect Proteins/*genetics/metabolism Ketones/pharmacology Pheromones/*pharmacology Promoter Regions, Genetic Quercetin/pharmacology Tanni;"
Notes:		"MedlineLi, Fen Ma, Kangsheng Chen, Xuewei Zhou, Jing-Jiang Gao, Xiwu eng Biotechnology and Biological Sciences Research Council/ Rothamsted Research/ 31330064/National Natural Science Foundation of China/ England 2018/05/26 Pest Manag Sci. 2019 Jan; 75(1):152-159. doi: 10.1002/ps.5081. Epub 2018 Aug 29"