| Title: | Whi3 mnemon association with endoplasmic reticulum membranes confines the memory of deceptive courtship to the yeast mother cell |
| Author(s): | Lau Y; Oamen HP; Grogg M; Parfenova I; Saarikangas J; Hannay R; Nichols RA; Hilvert D; Barral Y; Caudron F; |
| Address: | "School of Biological and Behavioural Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Laboratory of Organic Chemistry, ETH Zurich, Vladimir-Prelog-Weg, 8093 Zurich, Switzerland. Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg, 8093 Zurich, Switzerland. Helsinki Institute of Life Science HiLIFE, Viikinkaari 5, 00790 Helsinki, Finland; Faculty of Biological and Environmental Sciences, Viikinkaari 5, 00790 Helsinki, Finland; Neuroscience Center, University of Helsinki, Viikinkaari 5, 00790 Helsinki, Finland. School of Biological and Behavioural Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK; IGMM, Univ Montpellier, CNRS, Route de Mende, 34293 Montpellier, France. Electronic address: fabrice.caudron@igmm.cnrs.fr" |
| DOI: | 10.1016/j.cub.2022.01.002 |
| ISSN/ISBN: | 1879-0445 (Electronic) 0960-9822 (Print) 0960-9822 (Linking) |
| Abstract: | "Prion-like proteins are involved in many aspects of cellular physiology, including cellular memory. In response to deceptive courtship, budding yeast escapes pheromone-induced cell-cycle arrest through the coalescence of the G1/S inhibitor Whi3 into a dominant, inactive super-assembly. Whi3 is a mnemon (Whi3(mnem)), a protein that conformational change maintains as a trait in the mother cell but is not inherited by the daughter cells. How the maintenance and asymmetric inheritance of Whi3(mnem) are achieved is unknown. Here, we report that Whi3(mnem) is closely associated with endoplasmic reticulum (ER) membranes and is retained in the mother cell by the lateral diffusion barriers present at the bud neck. Strikingly, barrier defects made Whi3(mnem) propagate in a mitotically stable, prion-like manner. The amyloid-forming glutamine-rich domain of Whi3 was required for both mnemon and prion-like behaviors. Thus, we propose that Whi3(mnem) is in a self-templating state, lending temporal maintenance of memory, whereas its association with the compartmentalized membranes of the ER prevents infectious propagation to the daughter cells. These results suggest that confined self-templating super-assembly is a powerful mechanism for the long-term encoding of information in a spatially defined manner. Yeast courtship may provide insights on how individual synapses become potentiated in neuronal memory" |
| Keywords: | Courtship Endoplasmic Reticulum/metabolism RNA-Binding Proteins/metabolism *Saccharomyces cerevisiae/metabolism *Saccharomyces cerevisiae Proteins/genetics/metabolism Stem Cells/metabolism;Neuroscience; |
| Notes: | "MedlineLau, Yasmin Oamen, Henry Patrick Grogg, Marcel Parfenova, Iuliia Saarikangas, Juha Hannay, Robin Nichols, Richard Alan Hilvert, Donald Barral, Yves Caudron, Fabrice eng BB/S001204/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2022/01/28 Curr Biol. 2022 Mar 14; 32(5):963-974.e7. doi: 10.1016/j.cub.2022.01.002. Epub 2022 Jan 31" |
