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Cells

Title:		Functional Analysis of Pheromone Biosynthesis Activating Neuropeptide Receptor Isoforms in Maruca vitrata
Author(s):		Cha WH; Kim B; Lee DW;
Address:		"Department of SmartBio, Kyungsung University, Busan 48434, Republic of Korea. Metabolomics Research Center for Functional Materials, Kyungsung University, Busan 48434, Republic of Korea"
Journal Title:		Cells
Year:		2023
Volume:		20230517
Issue:		10
Page Number:		-
DOI:		10.3390/cells12101410
ISSN/ISBN:		2073-4409 (Electronic) 2073-4409 (Linking)
Abstract:		"Insect sex pheromones are volatile chemicals that induce mating behavior between conspecific individuals. In moths, sex pheromone biosynthesis is initiated when pheromone biosynthesis-activating neuropeptide (PBAN) synthesized in the suboesophageal ganglion binds to its receptor on the epithelial cell membrane of the pheromone gland. To investigate the function of PBAN receptor (PBANR), we identified two PBANR isoforms, MviPBANR-B and MviPBANR-C, in the pheromone glands of Maruca vitrata. These two genes belong to G protein-coupled receptors (GPCRs) and have differences in the C-terminus but share a 7-transmembrane region and GPCR family 1 signature. These isoforms were expressed in all developmental stages and adult tissues. MviPBANR-C had the highest expression level in pheromone glands among the examined tissues. Through in vitro heterologous expression in HeLa cell lines, only MviPBANR-C-transfected cells responded to MviPBAN (>/=5 microM MviPBAN), inducing Ca(2+) influx. Sex pheromone production and mating behavior were investigated using gas chromatography and a bioassay after MviPBANR-C suppression by RNA interference, which resulted in the major sex pheromone component, E10E12-16:Ald, being quantitatively reduced compared to the control, thereby decreasing the mating rate. Our findings indicate that MviPBANR-C is involved in the signal transduction of sex pheromone biosynthesis in M. vitrata and that the C-terminal tail plays an important role in its function"
Keywords:		"Humans Animals *Sex Attractants/metabolism HeLa Cells Amino Acid Sequence Receptors, Neuropeptide/genetics/metabolism *Moths/genetics Protein Isoforms/metabolism Maruca vitrata PBAN receptor RNA interference confocal microscopy gas chromatography sex pher;"
Notes:		"MedlineCha, Wook Hyun Kim, Boyun Lee, Dae-Weon eng Research Support, Non-U.S. Gov't Switzerland 2023/07/06 Cells. 2023 May 17; 12(10):1410. doi: 10.3390/cells12101410"