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Science

Title:		Tip20p prohibits back-fusion of COPII vesicles with the endoplasmic reticulum
Author(s):		Kamena F; Spang A;
Address:		"Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Spemannstrasse 39, D-72076 Tubingen, Germany"
Journal Title:		Science
Year:		2004
Volume:		304
Issue:		5668
Page Number:		286 - 289
DOI:		10.1126/science.1095049
ISSN/ISBN:		1095-9203 (Electronic) 0036-8075 (Linking)
Abstract:		"Directionality in intracellular trafficking is essential to ensure the correct localization of proteins along the secretory pathway. Here, we found evidence for an active mechanism that prohibited back-fusion of de novo-generated vesicles with their donor compartment. Tip20p is a peripheral membrane protein implicated in consumption of COPI vesicles at the endoplasmic reticulum. However, a specific mutant of TIP20 did not interfere with COPII vesicle generation but allowed these vesicles to fuse back to the endoplasmic reticulum, a process that does not occur normally in the cell"
Keywords:		COP-Coated Vesicles/*physiology/ultrastructure Carrier Proteins/genetics/*physiology Endoplasmic Reticulum/metabolism/*physiology/ultrastructure Glycoproteins/genetics/*physiology Golgi Apparatus/metabolism Intracellular Membranes/physiology/ultrastructur;
Notes:		"MedlineKamena, Faustin Spang, Anne eng Research Support, Non-U.S. Gov't 2004/04/10 Science. 2004 Apr 9; 304(5668):286-9. doi: 10.1126/science.1095049"