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Sci Rep

Title:		Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones
Author(s):		Cattaneo AM; Gonzalez F; Bengtsson JM; Corey EA; Jacquin-Joly E; Montagne N; Salvagnin U; Walker WB; Witzgall P; Anfora G; Bobkov YV;
Address:		"Fondazione Edmund Mach, Research and Innovation Centre/DASB, Agricultural Entomology, San Michele all'Adige, TN, Italy. University of Florida, Whitney Laboratory, Center for Smell and Taste, and McKnight Brain Institute, Gainesville, FL, USA. Universita degli Studi di Milano, Department of Food, Nutritional and Environmental Sciences, Milan, Italy. Universita di Cagliari, Dipartimento di Scienze Biomediche, Monserrato, CA, Italy. Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Unit, Alnarp, Sweden. Stockholm University, Department of Zoology, Stockholm, Sweden. INRA, Institute of Ecology and Environmental Sciences (iEES-Paris), Versailles, France. Sorbonne Universites, UPMC Univ Paris 06, iEES-Paris, Paris, France. Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Jena, Germany"
Journal Title:		Sci Rep
Year:		2017
Volume:		20170124
Issue:
Page Number:		41105 -
DOI:		10.1038/srep41105
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Olfaction plays a dominant role in the mate-finding and host selection behaviours of the codling moth (Cydia pomonella), an important pest of apple, pear and walnut orchards worldwide. Antennal transcriptome analysis revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ORs) CpomOR1, CpomOR3 and CpomOR6a, which belong to the pheromone receptor (PR) lineage, and the co-receptor (CpomOrco). Using heterologous expression, in both Drosophila olfactory sensory neurones and in human embryonic kidney cells, together with electrophysiological recordings and calcium imaging, we characterize the basic physiological and pharmacological properties of these receptors and demonstrate that they form functional ionotropic receptor channels. Both the homomeric CpomOrco and heteromeric CpomOrco + OR complexes can be activated by the common Orco agonists VUAA1 and VUAA3, as well as inhibited by the common Orco antagonists amiloride derivatives. CpomOR3 responds to the plant volatile compound pear ester ethyl-(E,Z)-2,4-decadienoate, while CpomOR6a responds to the strong pheromone antagonist codlemone acetate (E,E)-8,10-dodecadien-1-yl acetate. These findings represent important breakthroughs in the deorphanization of codling moth pheromone receptors, as well as more broadly into insect ecology and evolution and, consequently, for the development of sustainable pest control strategies based on manipulating chemosensory communication"
Keywords:		Animals Cell Line Decanoates/*pharmacology Dodecanol/analogs & derivatives Drosophila/drug effects Female Gene Expression Profiling Humans Insect Proteins/*agonists Male Moths/*metabolism Olfactory Receptor Neurons/drug effects/metabolism Pheromones/*phar;
Notes:		"MedlineCattaneo, Alberto Maria Gonzalez, Francisco Bengtsson, Jonas M Corey, Elizabeth A Jacquin-Joly, Emmanuelle Montagne, Nicolas Salvagnin, Umberto Walker, William B Witzgall, Peter Anfora, Gianfranco Bobkov, Yuriy V eng Research Support, Non-U.S. Gov't England 2017/01/25 Sci Rep. 2017 Jan 24; 7:41105. doi: 10.1038/srep41105"