| Title: | Pseudomonas strains naturally associated with potato plants produce volatiles with high potential for inhibition of Phytophthora infestans |
| Author(s): | Hunziker L; Bonisch D; Groenhagen U; Bailly A; Schulz S; Weisskopf L; |
| Address: | "Ecology of Noxious and Beneficial Organisms, Institute for Sustainability Sciences, Agroscope, Zurich, Switzerland. Institute of Organic Chemistry, Technische Universitat Braunschweig, Braunschweig, Germany. Ecology of Noxious and Beneficial Organisms, Institute for Sustainability Sciences, Agroscope, Zurich, Switzerland laure.weisskopf@agroscope.admin.ch" |
| ISSN/ISBN: | 1098-5336 (Electronic) 0099-2240 (Print) 0099-2240 (Linking) |
| Abstract: | "Bacteria emit volatile organic compounds with a wide range of effects on bacteria, fungi, plants, and animals. The antifungal potential of bacterial volatiles has been investigated with a broad span of phytopathogenic organisms, yet the reaction of oomycetes to these volatile signals is largely unknown. For instance, the response of the late blight-causing agent and most devastating oomycete pathogen worldwide, Phytophthora infestans, to bacterial volatiles has not been assessed so far. In this work, we analyzed this response and compared it to that of selected fungal and bacterial potato pathogens, using newly isolated, potato-associated bacterial strains as volatile emitters. P. infestans was highly susceptible to bacterial volatiles, while fungal and bacterial pathogens were less sensitive. Cyanogenic Pseudomonas strains were the most active, leading to complete growth inhibition, yet noncyanogenic ones also produced antioomycete volatiles. Headspace analysis of the emitted volatiles revealed 1-undecene as a compound produced by strains inducing volatile-mediated P. infestans growth inhibition. Supplying pure 1-undecene to P. infestans significantly reduced mycelial growth, sporangium formation, germination, and zoospore release in a dose-dependent manner. This work demonstrates the high sensitivity of P. infestans to bacterial volatiles and opens new perspectives for sustainable control of this devastating pathogen" |
| Keywords: | "Antifungal Agents/*metabolism Molecular Sequence Data Phytophthora infestans/*drug effects/*growth & development Pseudomonas/*metabolism Sequence Analysis, DNA Solanum tuberosum/*microbiology Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlineHunziker, Lukas Bonisch, Denise Groenhagen, Ulrike Bailly, Aurelien Schulz, Stefan Weisskopf, Laure eng Research Support, Non-U.S. Gov't 2014/11/16 Appl Environ Microbiol. 2015 Feb; 81(3):821-30. doi: 10.1128/AEM.02999-14. Epub 2014 Nov 14" |
