| Title: | "Sex pheromone communication in an insect parasitoid, Campoletis chlorideae Uchida" |
| Author(s): | Guo H; Mo BT; Li GC; Li ZL; Huang LQ; Sun YL; Dong JF; Smith DP; Wang CZ; |
| Address: | "State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. Chinese Academy of Sciences Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China. College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471000, People's Republic of China. Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390-9111. Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9111" |
| ISSN/ISBN: | 1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking) |
| Abstract: | "Sex pheromones are pivotal for insect reproduction. However, the mechanism of sex pheromone communication remains enigmatic in hymenopteran parasitoids. Here we have identified the sex pheromone and elucidated the olfactory basis of sex pheromone communication in Campoletis chlorideae (Ichneumonidae), a solitary larval endoparasitoid of over 30 lepidopteran pests. Using coupled gas chromatography-electroantennogram detection, we identified two female-derived pheromone components, tetradecanal (14:Ald) and 2-heptadecanone (2-Hep) (1:4.6), eliciting strong antennal responses from males but weak responses from females. We observed that males but not females were attracted to both single components and the blend. The hexane-washed female cadavers failed to arouse males, and replenishing 14:Ald and 2-Hep could partially restore the sexual attraction of males. We further expressed six C. chlorideae male-biased odorant receptors in Drosophila T1 neurons and found that CchlOR18 and CchlOR47 were selectively tuned to 14:Ald and 2-Hep, respectively. To verify the biological significance of this data, we knocked down CchlOR18 and CchlOR47 individually or together in vivo and show that the attraction of C. chlorideae to their respective ligands was abolished. Moreover, the parasitoids defective in either of the receptors were less likely to court and copulate. Finally, we show that the sex pheromone and (Z)-jasmone, a potent female attractant, can synergistically affect behaviors of virgin males and virgin females and ultimately increase the parasitic efficiency of C. chlorideae. Our study provides new insights into the molecular mechanism of sex pheromone communication in C. chlorideae that may permit manipulation of parasitoid behavior for pest control" |
| Keywords: | "Male Animals *Sex Attractants Insecta Communication Pheromones *Receptors, Odorant Drosophila chemical communications parasitoids pheromone receptors sex pheromone;Neuroscience;" |
| Notes: | "MedlineGuo, Hao Mo, Bao-Tong Li, Guo-Cheng Li, Zi-Lin Huang, Ling-Qiao Sun, Ya-Lan Dong, Jun-Feng Smith, Dean P Wang, Chen-Zhu eng Research Support, Non-U.S. Gov't 2022/11/29 Proc Natl Acad Sci U S A. 2022 Dec 6; 119(49):e2215442119. doi: 10.1073/pnas.2215442119. Epub 2022 Nov 29" |
