| Title: | Scp160-dependent mRNA trafficking mediates pheromone gradient sensing and chemotropism in yeast |
| Author(s): | Gelin-Licht R; Paliwal S; Conlon P; Levchenko A; Gerst JE; |
| Address: | "Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel" |
| DOI: | 10.1016/j.celrep.2012.03.004 |
| ISSN/ISBN: | 2211-1247 (Electronic) 2211-1247 (Print) |
| Abstract: | "mRNAs encoding polarity and secretion factors (POLs) target the incipient bud site in yeast for localized translation during division. In pheromone-treated cells we now find that these mRNAs are also localized to the yeast-mating projection (shmoo) tip. However, in contrast to the budding program, neither the She2 nor She3 proteins are involved. Instead, the Scp160 RNA-binding protein binds POL and mating pathway mRNAs and regulates their spatial distribution in a Myo4- and cortical ER-dependent fashion. RNA binding by Scp160 is stimulated by activation of Gpa1, the G protein alpha subunit regulated by the pheromone receptor, and is required for pheromone gradient sensing, as well as subsequent chemotropic growth and cell-cell mating. These effects are incurred independently of obvious changes in translation; thus, mRNA trafficking is required for chemotropism and completion of the mating program. This is, to our knowledge, the first demonstration of ligand-activated RNA targeting in the development of a simple eukaryote" |
| Keywords: | "Adaptor Proteins, Signal Transducing/physiology Cell Division/physiology Cell Polarity/physiology Chemotaxis/*physiology GTP-Binding Protein alpha Subunits/physiology Myosin Heavy Chains/physiology Myosin Type V/physiology Pheromones/*physiology RNA, Mess;" |
| Notes: | "MedlineGelin-Licht, Rita Paliwal, Saurabh Conlon, Patrick Levchenko, Andre Gerst, Jeffrey E eng GM084332/GM/NIGMS NIH HHS/ U54 RR020839/RR/NCRR NIH HHS/ R01 GM084332/GM/NIGMS NIH HHS/ R01 GM084332-04/GM/NIGMS NIH HHS/ RR020839/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2012/07/27 Cell Rep. 2012 May 31; 1(5):483-94. doi: 10.1016/j.celrep.2012.03.004. Epub 2012 Apr 20" |
