| Title: | Phytohormone responses in pepper (Capsicum annuum L.) leaves under a high density of aphid infestation |
| Author(s): | Florencio-Ortiz V; Novak O; Casas JL; |
| Address: | "Unidad Asociada IPAB (UA-CSIC), Instituto Universitario de Investigacion CIBIO (Centro Iberoamericano de la Biodiversidad), University of Alicante, Alicante, Spain. Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Palacky University, Olomouc, CZ-78371, Czech Republic" |
| ISSN/ISBN: | 1399-3054 (Electronic) 0031-9317 (Linking) |
| Abstract: | "The time course response of selected phytohormones has been evaluated in sweet pepper plants (Capsicum annuum L.) submitted to a high density (200 aphids/plant) of aphid (Myzus persicae Sulzer) infestation. Abscisic acid (ABA), salicylic acid (SA), indole-3-acetic acid (IAA), and jasmonates (JAs), including jasmonic acid (JA), jasmonoyl-l-isoleucine (JA-Ile), and cis-OPDA have been simultaneously identified and quantitated by UHPLC-MS/MS in pepper leaf tissue harvested at 3, 8 hours post-infestation (hpi), 1, 2, 4 and 7 days post-infestation (dpi). Infested plants showed a reduction in stem length at 7 dpi and in the number of leaves and leaf width from 4 dpi onwards. JA and JA-Ile significantly increased very early (from 3 hpi) while SA only accumulated at 7 dpi. Despite the high density of infestation, the aphid-induced accumulation of JAs was much lower than the burst typically induced by chewing herbivores. On the other side, ABA peaked in aphid-infested plants at 2 and 4 dpi, while IAA content did not change significantly at any time point. Growth inhibition may be partially explained by the high levels of JAs found in aphid-infested plants. The possibility that the obtained results support the hypothesis of the aphid manipulation of plant metabolism is discussed" |
| Keywords: | Animals *Aphids *Capsicum Plant Growth Regulators Plant Leaves Tandem Mass Spectrometry; |
| Notes: | "MedlineFlorencio-Ortiz, Victoria Novak, Ondrej Casas, Jose L eng Project CGL2016-79054-R/Ministerio de Economia y Competitividad of Spain/ NPU I program with project LO1204/Ministry of Education, Youth and Sports of the Czech Republic/ grant UAFPU2013-5793/Universidad de Alicante/ Denmark 2020/08/15 Physiol Plant. 2020 Dec; 170(4):519-527. doi: 10.1111/ppl.13188. Epub 2020 Sep 7" |
