| Title: | Quantitative Trait Locus Analysis of Mating Behavior and Male Sex Pheromones in Nasonia Wasps |
| Author(s): | Diao W; Mousset M; Horsburgh GJ; Vermeulen CJ; Johannes F; van de Zande L; Ritchie MG; Schmitt T; Beukeboom LW; |
| Address: | "Evolutionary Genetics, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen 9700CC, The Netherlands w.diao@rug.nl l.w.beukeboom@rug.nl. Evolutionary Genetics, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen 9700CC, The Netherlands. Natural Environment Research Council Bio-Molecular Analysis Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, United Kingdom. Evolutionary Genetics, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen 9700CC, The Netherlands Department of Pulmonary Diseases, University Medical Center Groningen, Groningen 9700 RB, The Netherlands. Population Epigenetics and Epigenomics, Department of Plant Sciences, Technical University Munich, 85354, Freising, Germany Institute for Advanced Study, Technical University Munich, 85748, Garching, Germany. School of Biology, University of St. Andrews, St. Andrews KY16 9TH, United Kingdom. Department of Animal Ecology and Tropical Biology, Biocenter, University of Wurzburg, Wurzburg 97074, Germany" |
| ISSN/ISBN: | 2160-1836 (Electronic) 2160-1836 (Linking) |
| Abstract: | "A major focus in speciation genetics is to identify the chromosomal regions and genes that reduce hybridization and gene flow. We investigated the genetic architecture of mating behavior in the parasitoid wasp species pair Nasonia giraulti and Nasonia oneida that exhibit strong prezygotic isolation. Behavioral analysis showed that N. oneida females had consistently higher latency times, and broke off the mating sequence more often in the mounting stage when confronted with N. giraulti males compared with males of their own species. N. oneida males produce a lower quantity of the long-range male sex pheromone (4R,5S)-5-hydroxy-4-decanolide (RS-HDL). Crosses between the two species yielded hybrid males with various pheromone quantities, and these males were used in mating trials with females of either species to measure female mate discrimination rates. A quantitative trait locus (QTL) analysis involving 475 recombinant hybrid males (F2), 2148 reciprocally backcrossed females (F3), and a linkage map of 52 equally spaced neutral single nucleotide polymorphism (SNP) markers plus SNPs in 40 candidate mating behavior genes revealed four QTL for male pheromone amount, depending on partner species. Our results demonstrate that the RS-HDL pheromone plays a role in the mating system of N. giraulti and N. oneida, but also that additional communication cues are involved in mate choice. No QTL were found for female mate discrimination, which points at a polygenic architecture of female choice with strong environmental influences" |
| Keywords: | "Animals Chromosome Mapping Female *Genetic Association Studies Genetic Linkage Genotype Male Phenotype Pheromones/*genetics Polymorphism, Single Nucleotide *Quantitative Trait Loci Sex Attractants/*genetics *Sexual Behavior, Animal Wasps/*genetics Nasonia;" |
| Notes: | "MedlineDiao, Wenwen Mousset, Mathilde Horsburgh, Gavin J Vermeulen, Cornelis J Johannes, Frank van de Zande, Louis Ritchie, Michael G Schmitt, Thomas Beukeboom, Leo W eng Research Support, Non-U.S. Gov't England 2016/05/14 G3 (Bethesda). 2016 Jun 1; 6(6):1549-62. doi: 10.1534/g3.116.029074" |
