« Previous AbstractExogenous nitrogen enhances poplar resistance to leaf herbivory and pathogen infection after exposure to soil cadmium stress    Next Abstract"Integration of transcriptome and proteome reveals molecular mechanisms underlying stress responses of the cutworm, Spodoptera litura, exposed to different levels of lead (Pb)" »

J Econ Entomol

Title:		Functional Characterization of Two Antenna-Enriched Odorant-Binding Proteins From Bactrocera minax (Diptera: Tephritidae)
Author(s):		Chen J; Yang L; Tian XL; Gui LY; Wang FL; Zhang GH;
Address:		"College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, P. R. China. College of Life Science, Yangtze University, Jingzhou, Hubei 434025, P. R. China"
Journal Title:		J Econ Entomol
Year:		2021
Volume:		114
Issue:		6
Page Number:		2361 - 2369
DOI:		10.1093/jee/toab199
ISSN/ISBN:		1938-291X (Electronic) 0022-0493 (Linking)
Abstract:		"Olfaction is of great significance for insect mate-seeking and host-locating behaviors. Insect odorant-binding proteins (OBPs), especially those antenna-enriched OBPs, are thought to discriminate, capture and transport odorant molecules to olfactory receptors, but this has not been fully clarified in Bactrocera minax (Enderlein), an economically important pest of citrus crops. Our previous studies showed that seven OBP genes (BminOBP1-7) were identified from B. minax adults via a head transcriptome analysis, of which only BminOBP3 and 6 were highly expressed in antennae, suggesting an olfactory role. To confirm their functions, here, BminOBP3 and 6 were cloned, expressed in Escherichia coli cells. Binding properties of the recombinant BminOBPs with 13 volatiles, most of which can elicit a significant behavioral response from B. minax adults, were determined by fluorescent competitive binding assays. The results showed that Both BminOBP3 and 6 exhibited a remarkable selectivity towards the 13 ligands tested. BminOBP3 displayed strong binding affinity only with undecanol. BminOBP6 demonstrated strong binding affinity with undecanol and limonene among 13 ligands tested. Undecanol is believed to be main sex pheromone component of B. minax. Limonene is an important volatile compound enriched in citrus fruits. Taken together, we concluded that BminOBP3 and 6 may play a prominent role in the process of B. minax mate-seeking and host-locating behaviors through recognizing and transporting these volatiles. It is conceivable that this study will increase our molecular understanding of B. minax olfaction, facilitating the development of OBP-based behavioral interference that is potentially useful for the integrated management of B. minax"
Keywords:		"Animals Carrier Proteins Insect Proteins/genetics/metabolism Odorants *Receptors, Odorant/genetics/metabolism *Tephritidae/genetics Bactrocera minax binding assay host volatile odorant-binding protein;"
Notes:		"MedlineChen, Jian Yang, Ling Tian, Xiao-Li Gui, Lian-You Wang, Fu-Lian Zhang, Guo-Hui eng Research Support, Non-U.S. Gov't England 2021/10/21 J Econ Entomol. 2021 Dec 6; 114(6):2361-2369. doi: 10.1093/jee/toab199"