| Title: | "Volatile fingerprints of seeds of four species indicate the involvement of alcoholic fermentation, lipid peroxidation, and Maillard reactions in seed deterioration during ageing and desiccation stress" |
| Author(s): | Colville L; Bradley EL; Lloyd AS; Pritchard HW; Castle L; Kranner I; |
| Address: | "Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK. l.colville@kew.org" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking) |
| Abstract: | "The volatile compounds released by orthodox (desiccation-tolerant) seeds during ageing can be analysed using gas chromatography-mass spectrometry (GC-MS). Comparison of three legume species (Pisum sativum, Lathyrus pratensis, and Cytisus scoparius) during artificial ageing at 60% relative humidity and 50 degrees C revealed variation in the seed volatile fingerprint between species, although in all species the overall volatile concentration increased with storage period, and changes could be detected prior to the onset of viability loss. The volatile compounds are proposed to derive from three main sources: alcoholic fermentation, lipid peroxidation, and Maillard reactions. Lipid peroxidation was confirmed in P. sativum seeds through analysis of malondialdehyde and 4-hydroxynonenal. Volatile production by ageing orthodox seeds was compared with that of recalcitrant (desiccation-sensitive) seeds of Quercus robur during desiccation. Many of the volatiles were common to both ageing orthodox seeds and desiccating recalcitrant seeds, with alcoholic fermentation forming the major source of volatiles. Finally, comparison was made between two methods of analysis; the first used a Tenax adsorbent to trap volatiles, whilst the second used solid phase microextraction to extract volatiles from the headspace of vials containing powdered seeds. Solid phase microextraction was found to be more sensitive, detecting a far greater number of compounds. Seed volatile analysis provides a non-invasive means of characterizing the processes involved in seed deterioration, and potentially identifying volatile marker compounds for the diagnosis of seed viability loss" |
| Keywords: | "Adsorption *Aging Aldehydes/metabolism Chromatography, High Pressure Liquid *Desiccation Fabaceae/chemistry/*physiology Fatty Acids/analysis/metabolism Fermentation Gas Chromatography-Mass Spectrometry/*methods Lipid Peroxidation Maillard Reaction Malondi;" |
| Notes: | "MedlineColville, Louise Bradley, Emma L Lloyd, Antony S Pritchard, Hugh W Castle, Laurence Kranner, Ilse eng Wellcome Trust/United Kingdom Comparative Study Research Support, Non-U.S. Gov't England 2012/11/24 J Exp Bot. 2012 Nov; 63(18):6519-30. doi: 10.1093/jxb/ers307" |
