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J Sci Food Agric

Title:		Optimisation of supercritical carbon dioxide extraction of essential oil of flowers of tea (Camellia sinensis L.) plants and its antioxidative activity
Author(s):		Chen Z; Mei X; Jin Y; Kim EH; Yang Z; Tu Y;
Address:		"Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China"
Journal Title:		J Sci Food Agric
Year:		2014
Volume:		20130701
Issue:		2
Page Number:		316 - 321
DOI:		10.1002/jsfa.6260
ISSN/ISBN:		1097-0010 (Electronic) 0022-5142 (Linking)
Abstract:		"BACKGROUND: To extract natural volatile compounds from tea (Camellia sinensis) flowers without thermal degradation and residue of organic solvents, supercritical fluid extraction (SFE) using carbon dioxide was employed to prepare essential oil of tea flowers in the present study. Four important parameters--pressure, temperature, static extraction time, and dynamic extraction time--were selected as independent variables in the SFE. RESULTS: The optimum extraction conditions were the pressure of 30 MPa, temperature of 50 degrees C, static time of 10 min, and dynamic time of 90 min. Based on gas chromatography-mass spectrometry analysis, 59 compounds, including alkanes (45.4%), esters (10.5%), ketones (7.1%), aldehydes (3.7%), terpenes (3.7%), acids (2.1%), alcohols (1.6%), ethers (1.3%) and others (10.3%) were identified in the essential oil of tea flowers. Moreover, the essential oil of tea flowers showed relatively stronger DPPH radical scavenging activity than essential oils of geranium and peppermint, although its antioxidative activity was weaker than those of essential oil of clove, ascorbic acid, tert-butylhydroquinone, and butylated hydroxyanisole. CONCLUSION: Essential oil of tea flowers using SFE contained many types of volatile compounds and showed considerable DPPH scavenging activity. The information will contribute to the future application of tea flowers as raw materials in health-care food and food flavour industries"
Keywords:		"Antioxidants/analysis/*pharmacology Biphenyl Compounds/metabolism Camellia sinensis/*chemistry Chromatography, Supercritical Fluid/methods Flowers/*chemistry Gas Chromatography-Mass Spectrometry Humans Oils, Volatile/chemistry/*pharmacology Picrates/metab;"
Notes:		"MedlineChen, Zhenchun Mei, Xin Jin, Yuxia Kim, Eun-Hye Yang, Ziyin Tu, Youying eng Research Support, Non-U.S. Gov't England 2013/06/12 J Sci Food Agric. 2014 Jan 30; 94(2):316-21. doi: 10.1002/jsfa.6260. Epub 2013 Jul 1"