| Title: | Positive Chemotaxis of the Entomopathogenic Nematode Steinernema australe (Panagrolaimorpha: Steinenematidae) towards High-Bush Blueberry (Vaccinium corymbosum) Root Volatiles |
| Author(s): | Ceballos R; Palma-Millanao R; Navarro PD; Urzua J; Alveal J; |
| Address: | "Laboratory of Insects Chemical Ecology, Instituto de Investigaciones Agropecuarias, INIA Quilamapu, Av. Vicente Mendez 515, Chillan 3800062, Chile. Laboratory of Insects Science, Instituto de Investigaciones Agropecuarias, INIA Carillanca, Km 10, Camino Cajon-Vilcun, Temuco 4800000, Chile. Vicerrectoria de Investigacion y Postgrado, Universidad de La Frontera, Temuco 4811230, Chile" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "The foraging behavior of the infective juveniles (IJs) of entomopathogenic nematodes (EPNs) relies on host-derived compounds, but in a tri-trophic context, herbivore-induced root volatiles act as signals enhancing the biological control of insect pests by recruiting EPNs. In southern Chile, the EPN Steinernema australe exhibits the potential to control the raspberry weevil, Aegorhinus superciliosus, a key pest of blueberry Vaccinium corymbosum. However, there is no information on the quality of the blueberry root volatile plume or the S. australe response to these chemicals as putative attractants. Here, we describe the root volatile profile of blueberries and the chemotaxis behavior of S. australe towards the volatiles identified from Vaccinium corymbosum roots, infested or uninfested with A. superciliosus larvae. Among others, we found linalool, alpha-terpineol, limonene, eucalyptol, 2-carene, 1-nonine, 10-undecyn-1-ol, and methyl salicylate in root volatiles and, depending on the level of the emissions, they were selected for bioassays. In the dose-response tests, S. australe was attracted to all five tested concentrations of methyl salicylate, 1-nonine, alpha-terpineol, and 2-carene, as well as to 100 microg mL(-1) of 10-undecyn-1-ol, 0.1 and 100 microg mL(-1) of linalool, and 100 microg mL(-1) of limonene, whereas eucalyptol elicited no attraction or repellency. These results suggest that some volatiles released from damaged roots attract S. australe and may have implications for the biocontrol of subterranean pests" |
| Keywords: | "Animals *Blueberry Plants Limonene Chemotaxis Eucalyptol *Rhabditida Larva/physiology *Weevils/physiology Pest Control, Biological/methods belowground interactions entomopathogenic nematode recruitment foraging behavior olfactometer root volatiles;" |
| Notes: | "MedlineCeballos, Ricardo Palma-Millanao, Ruben Navarro, Patricia D Urzua, Julio Alveal, Juan eng Fondef/ Concurso IDeA I+D 18I10005/Agencia Nacional de Investigacion y Desarrollo/ Switzerland 2023/07/14 Int J Mol Sci. 2023 Jun 23; 24(13):10536. doi: 10.3390/ijms241310536" |
