| Title: | Volatile Organic Compounds in Exhaled Air as Novel Marker for Disease Activity in Crohn's Disease: A Metabolomic Approach |
| Author(s): | Bodelier AG; Smolinska A; Baranska A; Dallinga JW; Mujagic Z; Vanhees K; van den Heuvel T; Masclee AA; Jonkers D; Pierik MJ; van Schooten FJ; |
| Address: | "*Department of Gastroenterology and Hepatology, Maastricht University Medical Center+, Maastricht, the Netherlands; daggerDepartment of Gastroenterology, Amphia Hospital, Breda, the Netherlands; double daggerDepartment of Toxicology, Research Institute NUTRIM, Maastricht University Medical Center+, Maastricht, the Netherlands; and section signTop Institute Food and Nutrition, Wageningen, the Netherlands" |
| DOI: | 10.1097/MIB.0000000000000436 |
| ISSN/ISBN: | 1536-4844 (Electronic) 1078-0998 (Linking) |
| Abstract: | "BACKGROUND: Disappearance of macroscopic mucosal inflammation predicts long-term outcome in Crohn's disease (CD). It can be assessed by ileocolonoscopy, which is, however, an invasive and expensive procedure. Disease activity indices do not correlate well with endoscopic activity and noninvasive markers have a low sensitivity in subgroups of patients. Volatile organic compounds (VOCs) in breath are of increasing interest as noninvasive markers. The aim of this study was to investigate whether VOCs can accurately differentiate between active CD and remission. METHODS: Patients participated in a 1-year follow-up study and Harvey-Bradshaw index, blood, fecal, and breath samples were collected at regular intervals. Patients were stratified into 2 groups: active (fecal calprotectin >250 microg/g) or inactive (Harvey-Bradshaw index <4, C-reactive protein <5 mg/L, and fecal calprotectin <100 microg/g) disease. Breath samples were analyzed by gas chromatography-time-of-flight mass spectrometry. Random forest analyses were used to find the most discriminatory VOCs. RESULTS: Eight hundred thirty-five breath-o-grams were measured, 140 samples were assigned as active, 135 as inactive disease, and 110 samples of healthy controls. A set of 10 discriminatory VOCs correctly predicted active CD in 81.5% and remission in 86.4% (sensitivity 0.81, specificity 0.80, AUC 0.80). These VOCs were combined into a single disease activity score that classified disease activity in more than 60% of the previously undetermined individuals. CONCLUSIONS: We showed that VOCs can separate healthy controls and patients with active CD and CD in remission in a real-life cohort. Analysis of exhaled air is an interesting new noninvasive application for monitoring mucosal inflammation in inflammatory bowel disease" |
| Keywords: | Adolescent Adult Biomarkers/*analysis Breath Tests/*methods Case-Control Studies Crohn Disease/*diagnosis/*metabolism Female Follow-Up Studies Humans Male *Metabolomics Prognosis Prospective Studies *Severity of Illness Index Volatile Organic Compounds/*a; |
| Notes: | "MedlineBodelier, Alexander G L Smolinska, Agnieszka Baranska, Agnieszka Dallinga, Jan W Mujagic, Zlatan Vanhees, Kimberly van den Heuvel, Tim Masclee, Ad A M Jonkers, Daisy Pierik, Marie J van Schooten, Frederik J eng Research Support, Non-U.S. Gov't England 2015/07/23 Inflamm Bowel Dis. 2015 Aug; 21(8):1776-85. doi: 10.1097/MIB.0000000000000436" |
