« Previous AbstractPositive and negative regulatory elements control expression of the yeast retrotransposon Ty3    Next AbstractThe profile of volatile compounds in the outer and inner parts of broiled pork neck is strongly influenced by the acetic-acid marination conditions »

Proc Natl Acad Sci U S A

Title:		Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster
Author(s):		Bilen J; Atallah J; Azanchi R; Levine JD; Riddiford LM;
Address:		"Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2013
Volume:		20131021
Issue:		45
Page Number:		18321 - 18326
DOI:		10.1073/pnas.1318119110
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met(27) females delayed the onset of mating, whereas lack of Gce had little effect. The Met(27) females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met(27) null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce(2.5k) null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating"
Keywords:		"Analysis of Variance Animals Corpora Allata/metabolism Drosophila melanogaster/*physiology Female Hydrocarbons/metabolism Juvenile Hormones/deficiency/*metabolism Male Phenothiazines/metabolism Sex Attractants/*biosynthesis Sexual Behavior, Animal/*physio;"
Notes:		"MedlineBilen, Julide Atallah, Jade Azanchi, Reza Levine, Joel D Riddiford, Lynn M eng Canadian Institutes of Health Research/Canada Comparative Study Research Support, Non-U.S. Gov't 2013/10/23 Proc Natl Acad Sci U S A. 2013 Nov 5; 110(45):18321-6. doi: 10.1073/pnas.1318119110. Epub 2013 Oct 21"